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Literature summary for 1.4.1.2 extracted from

  • Sharkey, M.A.; Gori, A.; Capone, M.; Engel, P.C.
    Reversal of the extreme coenzyme selectivity of Clostridium symbiosum glutamate dehydrogenase (2012), FEBS J., 279, 3003-3009.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and mutant enzymes [Clostridium] symbiosum

Protein Variants

Protein Variants Comment Organism
D263K site-directed mutagenesis, the mutant shows altered cofactor specificity compared to the wild-type enzyme [Clostridium] symbiosum
F238S/P262S site-directed mutagenesis, the mutant shows altered cofactor specificity compared to the wild-type enzyme [Clostridium] symbiosum
F238S/P262S/D263K/N290G site-directed mutagenesis, the mutant shows altered cofactor specificity compared to the wild-type enzyme [Clostridium] symbiosum
F238S/P262S/N290G site-directed mutagenesis, the mutant shows altered cofactor specificity compared to the wild-type enzyme [Clostridium] symbiosum
F238S?P262S?D263K site-directed mutagenesis, the mutant shows complete reversal in coenzyme selectivity from NAD(H) to NADP(H) with retention of high levels of catalytic activity for the second coenzyme [Clostridium] symbiosum
N290G site-directed mutagenesis, the mutant shows altered cofactor specificity compared to the wild-type enzyme [Clostridium] symbiosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of recombinant wild-type and mutant enzymes with NADH/NAD+ and NADPH/NADP+, overview [Clostridium] symbiosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD+ [Clostridium] symbiosum
-
2-oxoglutarate + NH3 + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
[Clostridium] symbiosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes by affinity chromatography purification, followed by gel filtration [Clostridium] symbiosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
[Clostridium] symbiosum 2-oxoglutarate + NH3 + NADH + H+
-
r

Subunits

Subunits Comment Organism
More structure modelling of cofactor-bound enzyme, overview [Clostridium] symbiosum

Synonyms

Synonyms Comment Organism
GDH
-
[Clostridium] symbiosum
NAD+-dependent GDH
-
[Clostridium] symbiosum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at [Clostridium] symbiosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at [Clostridium] symbiosum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
[Clostridium] symbiosum
NADH
-
[Clostridium] symbiosum