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Literature summary for 1.4.1.2 extracted from
- Gene cloning, heterologous overexpression and optimized refolding of the NAD-glutamate dehydrogenase from Haloferax mediterranei (2006), Extremophiles, 10, 105-115.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ligated to NdeI/BamHI digested pET3a, expression in Escherichia coli BL21 (DE3) | Haloferax mediterranei |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | activity with KCl slightly higher than with NaCl | Haloferax mediterranei |  |
| NaCl | - |
Haloferax mediterranei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47950 | - |
x * 54000, SDS-PAGE, x * 47950, sequence analysis | Haloferax mediterranei |
| 54000 | - |
x * 54000, SDS-PAGE, x * 47950, sequence analysis | Haloferax mediterranei |
| 290000 | - |
recombinant NAD-GDH | Haloferax mediterranei |
| 310000 | - |
wild-type NAD-GDH | Haloferax mediterranei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloferax mediterranei | Q977U6 | strain R4 (ATCC 33500) | - |
| Haloferax mediterranei R-4 / ATCC 33500 | Q977U6 | strain R4 (ATCC 33500) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration, native enzyme 232fold purified, recombinant enzyme 5.4fold purified | Haloferax mediterranei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 89.3 | - |
purified recombinant enzyme | Haloferax mediterranei |
| 104 | - |
purified native enzyme | Haloferax mediterranei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NH3 + NADH | - |
Haloferax mediterranei | L-glutamate + H2O + NAD+ | - |
r | |
| 2-oxoglutarate + NH3 + NADH | - |
Haloferax mediterranei R-4 / ATCC 33500 | L-glutamate + H2O + NAD+ | - |
r | |
| L-glutamate + H2O + NAD+ | - |
Haloferax mediterranei | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
| L-glutamate + H2O + NAD+ | - |
Haloferax mediterranei R-4 / ATCC 33500 | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, SDS-PAGE, x * 47950, sequence analysis | Haloferax mediterranei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDH, NAD-dependent | - |
Haloferax mediterranei |
| NAD-GDH | - |
Haloferax mediterranei |
| NAD-glutamate dehydrogenase | - |
Haloferax mediterranei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Haloferax mediterranei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Haloferax mediterranei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 9.5 | - |
Haloferax mediterranei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NADPH | Haloferax mediterranei | |
| NAD+ | - |
Haloferax mediterranei | |
| NADH | - |
Haloferax mediterranei | |
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