Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
G82K | dramatically switches to increased specificity for oxaloacetate, 280fold higher than those for 2-oxoglutarate | Bacillus subtilis |
G82R | specific activity not drastically altered compared to the wild-type | Bacillus subtilis |
K80R | specific activity not drastically altered compared to the wild-type | Bacillus subtilis |
M101K | specific activity not drastically altered compared to the wild-type | Bacillus subtilis |
M101S | dramatically switches to increased specificity for oxaloacetate, 495fold higher than those for 2-oxoglutarate | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
NADH | wild-type | Bacillus subtilis | |
0.091 | - |
NADH | mutant M101S | Bacillus subtilis | |
0.095 | - |
NADH | mutant G82K | Bacillus subtilis | |
0.65 | - |
2-oxoglutarate | wild-type | Bacillus subtilis | |
2.27 | - |
oxaloacetate | mutant M101S | Bacillus subtilis | |
4.16 | - |
oxaloacetate | mutant G82K | Bacillus subtilis | |
100 | - |
2-oxoglutarate | mutant M101S | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.012 | - |
2-oxoglutarate, mutant M101K | Bacillus subtilis |
0.014 | - |
2-oxoglutarate, mutant K80R | Bacillus subtilis |
0.019 | - |
2-oxoglutarate, mutant G82R and M101S | Bacillus subtilis |
0.026 | - |
2-oxoglutarate, mutant G82K | Bacillus subtilis |
0.21 | - |
on L-aspartate, mutant M101S | Bacillus subtilis |
0.39 | - |
on L-aspartate, mutant G82K | Bacillus subtilis |
440 | - |
2-oxoglutarate, wild-type | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylvalerate + NADPH + NH3 | very low specificity of wild-type | Bacillus subtilis | L-isoleucine + NADP+ + H2O | - |
? | |
2-oxo-iso-caproate + NADPH + NH3 | very low specificity of wild-type | Bacillus subtilis | L-norleucine + NADP+ + H2O | - |
? | |
2-oxo-iso-valerate + NADPH + NH3 | low specificity of wild-type | Bacillus subtilis | L-leucine + NADP+ + H2O | - |
? | |
2-oxoglutarate + NADPH + NH3 | wild-type enzyme highly specific for 2-oxoglutarate | Bacillus subtilis | L-glutamate + NADP+ + H2O | - |
r | |
beta-phenylpyruvate + NADPH + NH3 | low specificity of wild-type | Bacillus subtilis | L-phenylalanine + NADP+ + H2O | - |
? | |
additional information | no specificity of wild-type for 2-ketohexonoate | Bacillus subtilis | ? | - |
? | |
oxaloacetate + NADPH + NH3 | very low specificity of wild-type | Bacillus subtilis | L-aspartate + NADP+ + H2O | - |
? | |
p-hydroxyphenylpyruvate + NADPH + NH3 | very low specificity of wild-type | Bacillus subtilis | L-tyrosine + NADP+ + H2O | - |
? | |
pyruvate + NADPH + NH3 | very low specificity of wild-type | Bacillus subtilis | L-alanine + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutamate dehydrogenase | - |
Bacillus subtilis |
NAD+-dependent GluDH | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
oxaloacetate | mutant M101S | Bacillus subtilis | |
0.012 | - |
2-oxoglutarate | mutant M101S | Bacillus subtilis | |
0.013 | - |
2-oxoglutarate | mutant G82K | Bacillus subtilis | |
3.45 | - |
oxaloacetate | mutant G82K | Bacillus subtilis | |
5.68 | - |
oxaloacetate | mutant M101S | Bacillus subtilis | |
344 | - |
2-oxoglutarate | wild-type | Bacillus subtilis |