Activating Compound | Comment | Organism | Structure |
---|---|---|---|
heating | at 90°C the activity of the recombinant enzyme increases to a level comparable to that of the native enzyme | Pyrobaculum islandicum | |
Urea | at 5 M the activity of the recombinant enzyme increases to a level comparable to that of the native enzyme, urea-induced activation of recombinant GDH is irreversible | Pyrobaculum islandicum |
Application | Comment | Organism |
---|---|---|
additional information | subunit rearrangement, i.e., a change in the quaternary structure of the hexameric recombinant GDH, is essential for activation of the enzyme | Pyrobaculum islandicum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli (DE3)-codon plus-RIL cells | Pyrobaculum islandicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
NAD+ | recombinant, urea-activated enzyme | Pyrobaculum islandicum | |
0.019 | - |
NAD+ | native enzyme | Pyrobaculum islandicum | |
0.021 | - |
NAD+ | recombinant, heat-activated enzyme | Pyrobaculum islandicum | |
0.16 | - |
L-glutamate | native enzyme | Pyrobaculum islandicum | |
0.16 | - |
L-glutamate | recombinant, heat-activated enzyme | Pyrobaculum islandicum | |
0.18 | - |
L-glutamate | recombinant, urea-activated enzyme | Pyrobaculum islandicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
6 * 47000, SDS-PAGE | Pyrobaculum islandicum |
280000 | - |
recombinant enzyme, gel filtration | Pyrobaculum islandicum |
299000 | - |
recombinant, heat-activated enzyme, gel filtration | Pyrobaculum islandicum |
337000 | - |
recombinant, urea-activated enzyme, gel filtration | Pyrobaculum islandicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum islandicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrobaculum islandicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.51 | - |
native enzyme | Pyrobaculum islandicum |
3.54 | - |
recombinant, urea-activated enzyme | Pyrobaculum islandicum |
3.65 | - |
recombinant, heat-activated enzyme | Pyrobaculum islandicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | - |
Pyrobaculum islandicum | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 47000, SDS-PAGE | Pyrobaculum islandicum |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Pyrobaculum islandicum |
glutamate dehydrogenase | - |
Pyrobaculum islandicum |