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Literature summary for 1.4.1.2 extracted from

  • Carrigan, J.B.; Coughlan, S.; Engel, P.C.
    Properties of the thermostable glutamate dehydrogenase of the mesophilic anaerobe Peptostreptoccus asaccharolyticus purified by a novel method after over-expression in an Escherichia coli host (2005), FEMS Microbiol. Lett., 244, 53-59.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Peptoniphilus asaccharolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0044
-
NADH pH 7, 25°C Peptoniphilus asaccharolyticus
0.103
-
2-oxoglutarate pH 7, 25°C Peptoniphilus asaccharolyticus
0.345
-
NAD+ pH 7, 25°C Peptoniphilus asaccharolyticus
1.64
-
NADPH pH 7, 25°C Peptoniphilus asaccharolyticus
3.7
-
NADP+ pH 7, 25°C Peptoniphilus asaccharolyticus
5.82
-
glutamate pH 7, 25°C Peptoniphilus asaccharolyticus
144
-
NH4+ pH 7, 25°C Peptoniphilus asaccharolyticus

Organism

Organism UniProt Comment Textmining
Peptoniphilus asaccharolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Peptoniphilus asaccharolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Peptoniphilus asaccharolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
Peptoniphilus asaccharolyticus L-glutamate + NADP+
-
?
L-glutamate + H2O + NAD+
-
Peptoniphilus asaccharolyticus 2-oxoglutarate + NH3 + NADH + H+
-
r
L-glutamate + H2O + NADP+
-
Peptoniphilus asaccharolyticus 2-oxoglutarate + NH3 + NADPH
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Peptoniphilus asaccharolyticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 40°C: about 50% of maximal activity, 80°C: about 40% of maximal activity Peptoniphilus asaccharolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
24 h, stable Peptoniphilus asaccharolyticus
72
-
half-life: 410 min Peptoniphilus asaccharolyticus
78
-
half-life: 50 min Peptoniphilus asaccharolyticus
82
-
half-life: 30 min Peptoniphilus asaccharolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
NADP+ pH 7, 25°C Peptoniphilus asaccharolyticus
6
-
NAD+ pH 7, 25°C Peptoniphilus asaccharolyticus
6.7
-
glutamate pH 7, 25°C Peptoniphilus asaccharolyticus
12
-
NADPH pH 7, 25°C Peptoniphilus asaccharolyticus
20.3
-
2-oxoglutarate pH 7, 25°C Peptoniphilus asaccharolyticus
20.4
-
NH4+ pH 7, 25°C Peptoniphilus asaccharolyticus
31.4
-
NADH pH 7, 25°C Peptoniphilus asaccharolyticus

Cofactor

Cofactor Comment Organism Structure
NAD+ highly specific for. The ratio for turnover number to Km-value is 405 times greater than for NADP+ Peptoniphilus asaccharolyticus
NADH the ratio for turnover number to Km-value is 1000fold greater than for NADPH Peptoniphilus asaccharolyticus
NADP+ highly specific for NAD+. The ratio for turnover number to Km-value for NAD+ is 405 times greater than for NADP+ Peptoniphilus asaccharolyticus
NADPH the ratio for turnover number to Km-value for NADH is 1000fold greater than that for NADPH Peptoniphilus asaccharolyticus