Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Peptoniphilus asaccharolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0044 | - |
NADH | pH 7, 25°C | Peptoniphilus asaccharolyticus | |
0.103 | - |
2-oxoglutarate | pH 7, 25°C | Peptoniphilus asaccharolyticus | |
0.345 | - |
NAD+ | pH 7, 25°C | Peptoniphilus asaccharolyticus | |
1.64 | - |
NADPH | pH 7, 25°C | Peptoniphilus asaccharolyticus | |
3.7 | - |
NADP+ | pH 7, 25°C | Peptoniphilus asaccharolyticus | |
5.82 | - |
glutamate | pH 7, 25°C | Peptoniphilus asaccharolyticus | |
144 | - |
NH4+ | pH 7, 25°C | Peptoniphilus asaccharolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Peptoniphilus asaccharolyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Peptoniphilus asaccharolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Peptoniphilus asaccharolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Peptoniphilus asaccharolyticus | L-glutamate + NADP+ | - |
? | |
L-glutamate + H2O + NAD+ | - |
Peptoniphilus asaccharolyticus | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | - |
Peptoniphilus asaccharolyticus | 2-oxoglutarate + NH3 + NADPH | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Peptoniphilus asaccharolyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | 40°C: about 50% of maximal activity, 80°C: about 40% of maximal activity | Peptoniphilus asaccharolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
24 h, stable | Peptoniphilus asaccharolyticus |
72 | - |
half-life: 410 min | Peptoniphilus asaccharolyticus |
78 | - |
half-life: 50 min | Peptoniphilus asaccharolyticus |
82 | - |
half-life: 30 min | Peptoniphilus asaccharolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
NADP+ | pH 7, 25°C | Peptoniphilus asaccharolyticus | |
6 | - |
NAD+ | pH 7, 25°C | Peptoniphilus asaccharolyticus | |
6.7 | - |
glutamate | pH 7, 25°C | Peptoniphilus asaccharolyticus | |
12 | - |
NADPH | pH 7, 25°C | Peptoniphilus asaccharolyticus | |
20.3 | - |
2-oxoglutarate | pH 7, 25°C | Peptoniphilus asaccharolyticus | |
20.4 | - |
NH4+ | pH 7, 25°C | Peptoniphilus asaccharolyticus | |
31.4 | - |
NADH | pH 7, 25°C | Peptoniphilus asaccharolyticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | highly specific for. The ratio for turnover number to Km-value is 405 times greater than for NADP+ | Peptoniphilus asaccharolyticus | |
NADH | the ratio for turnover number to Km-value is 1000fold greater than for NADPH | Peptoniphilus asaccharolyticus | |
NADP+ | highly specific for NAD+. The ratio for turnover number to Km-value for NAD+ is 405 times greater than for NADP+ | Peptoniphilus asaccharolyticus | |
NADPH | the ratio for turnover number to Km-value for NADH is 1000fold greater than that for NADPH | Peptoniphilus asaccharolyticus |