Literature summary for 1.4.1.2 extracted from

Kersten, M.A.S.H.; Muller, Y.; Baars, J.J.P.; Op den Camp, H.J.M.; Van der Drift, C.; Van Griensven, L.J.L.D.; Visser, J.; Schaap, P.J.
NAD+-dependent glutamate dehydrogenase of the edible mushroom Agaricus bisporus: biochemical and molecular characterization (1999), Mol. Gen. Genet., 261, 452-462.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.046	-	NAD+	-	Agaricus bisporus
0.06	-	NADH	-	Agaricus bisporus
6.5	-	NH4+	-	Agaricus bisporus
37.1	-	L-glutamate	-	Agaricus bisporus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
116000	-	4 * 116000, SDS-PAGE	Agaricus bisporus
474000	-	gel filtration	Agaricus bisporus

Organism

Organism	UniProt	Comment	Textmining
Agaricus bisporus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Agaricus bisporus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.8	-	-	Agaricus bisporus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxoglutarate + NH3 + NADH	-	Agaricus bisporus	L-glutamate + H2O + NAD+	-	r
2-oxoglutarate + NH4+ + NADPH	-	Agaricus bisporus	L-glutamate + NADP+	-	?
L-glutamate + H2O + NAD+	-	Agaricus bisporus	2-oxoglutarate + NH3 + NADH + H+	-	r

Subunits

Subunits	Comment	Organism
tetramer	4 * 116000, SDS-PAGE	Agaricus bisporus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Agaricus bisporus

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Release 2023.1 (January 2023)