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Literature summary for 1.4.1.2 extracted from
- Purification & properties of isozymes of glutamate dehydrogenase form Bacillus cereus T (1976), Indian J. Biochem. Biophys., 13, 344-346.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 165000 | 175000 | GDHA, gel filtration | Bacillus cereus |
| 240000 | 250000 | GDH B, gel filtration | Bacillus cereus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isoenzymes GDHA, GDHB | Bacillus cereus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bacillus cereus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4-5°C, 0.066 M phosphate buffer, pH 7.4 or 0.01 M arginine buffer, pH 9.5, 20 days, stable | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + H2O + NAD+ | - |
Bacillus cereus | 2-oxoglutarate + NH3 + NADH + H+ | - |
? |  |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 45 | - |
Bacillus cereus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Bacillus cereus | |
| NADH | - |
Bacillus cereus | |
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