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Literature summary for 1.4.1.19 extracted from

  • Matsui, D.; Okazaki, S.; Matsuda, M.; Asano, Y.
    Enhancement of stability of L-tryptophan dehydrogenase from Nostoc punctiforme ATCC29133 and its application to L-tryptophan assay (2015), J. Biotechnol., 196-197, 27-32 .
    View publication on PubMed
Search for more literature for 1.4.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Nostoc punctiforme

Protein Variants

Protein Variants Comment Organism
A234D the mutant shows higher specific activity and stability compared to the wild type enzyme Nostoc punctiforme
D168G the mutant shows higher specific activity and stability compared to the wild type enzyme Nostoc punctiforme
I296N the mutant shows wild type specific activity and higher stability compared to the wild type enzyme Nostoc punctiforme
L59F the mutant shows higher specific activity and stability compared to the wild type enzyme Nostoc punctiforme
L59F/D168G/A234D/I296N mutant with thermal stability whose specific activity and stability are higher than those of the wild type enzyme Nostoc punctiforme

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.194
-
 L-tryptophan mutant enzyme L59F, at pH 11.5 and 30°C Nostoc punctiforme
0.201
-
 L-tryptophan wild type enzyme, at pH 11.5 and 30°C Nostoc punctiforme
0.221
-
 L-tryptophan mutant enzyme D168G, at pH 11.5 and 30°C Nostoc punctiforme
0.228
-
 L-tryptophan mutant enzyme I296N, at pH 11.5 and 30°C Nostoc punctiforme
0.268
-
 L-tryptophan mutant enzyme L59F/D168G/A234D/I296N, at pH 11.5 and 30°C Nostoc punctiforme
0.288
-
 L-tryptophan mutant enzyme A234D, at pH 11.5 and 30°C Nostoc punctiforme

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(indol-3-yl)pyruvate + NH3 + NADH + H+ Nostoc punctiforme
-
 L-tryptophan + NAD+ + H2O
-
 r
(indol-3-yl)pyruvate + NH3 + NADH + H+ Nostoc punctiforme ATCC29133
-
 L-tryptophan + NAD+ + H2O
-
 r
L-tryptophan + NAD+ + H2O Nostoc punctiforme
-
 (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r
L-tryptophan + NAD+ + H2O Nostoc punctiforme ATCC29133
-
 (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Nostoc punctiforme W8CV45
-
-
Nostoc punctiforme ATCC29133 W8CV45
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Nostoc punctiforme

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(indol-3-yl)pyruvate + NH3 + NADH + H+
-
 Nostoc punctiforme L-tryptophan + NAD+ + H2O
-
 r
(indol-3-yl)pyruvate + NH3 + NADH + H+
-
 Nostoc punctiforme ATCC29133 L-tryptophan + NAD+ + H2O
-
 r
L-tryptophan + NAD+ + H2O
-
 Nostoc punctiforme (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r
L-tryptophan + NAD+ + H2O
-
 Nostoc punctiforme ATCC29133 (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Nostoc punctiforme

Synonyms

Synonyms Comment Organism
NAD+-dependent L-tryptophan dehydrogenase
-
 Nostoc punctiforme
TrpDH
-
 Nostoc punctiforme

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 the enzyme retains 19.3% activity after incubation at 4°C for 24 h Nostoc punctiforme

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Nostoc punctiforme
NADH
-
 Nostoc punctiforme