Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by EDTA (10 mM), KCl (100 mM) or DMSO (1% (v/v)) | Nostoc punctiforme |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Nostoc punctiforme |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(indol-3-yl)pyruvate | substrate inhibition | Nostoc punctiforme |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
(indol-3-yl)pyruvate | at pH 7.5 and 25°C | Nostoc punctiforme | |
0.025 | - |
NAD+ | at pH 11.0 and 25°C | Nostoc punctiforme | |
0.031 | - |
L-tryptophan | at pH 11.0 and 25°C | Nostoc punctiforme | |
0.038 | - |
NADH | at pH 7.5 and 25°C | Nostoc punctiforme | |
130 | - |
NH3 | at pH 7.5 and 25°C | Nostoc punctiforme |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not activated by metal ions | Nostoc punctiforme |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76100 | - |
gel filtration | Nostoc punctiforme |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(indol-3-yl)pyruvate + NH3 + NADH + H+ | Nostoc punctiforme | - |
L-tryptophan + NAD+ + H2O | - |
r | |
(indol-3-yl)pyruvate + NH3 + NADH + H+ | Nostoc punctiforme NIES-2108 | - |
L-tryptophan + NAD+ + H2O | - |
r | |
L-tryptophan + NAD+ + H2O | Nostoc punctiforme | the enzyme is highly specific for the deamination of amino acid L-tryptophan | (indol-3-yl)pyruvate + NH3 + NADH + H+ | - |
r | |
L-tryptophan + NAD+ + H2O | Nostoc punctiforme NIES-2108 | the enzyme is highly specific for the deamination of amino acid L-tryptophan | (indol-3-yl)pyruvate + NH3 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc punctiforme | - |
- |
- |
Nostoc punctiforme NIES-2108 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Nostoc punctiforme |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(indol-3-yl)pyruvate + NH3 + NADH + H+ | - |
Nostoc punctiforme | L-tryptophan + NAD+ + H2O | - |
r | |
(indol-3-yl)pyruvate + NH3 + NADH + H+ | - |
Nostoc punctiforme NIES-2108 | L-tryptophan + NAD+ + H2O | - |
r | |
L-phenylalanine + NAD+ + H2O | 23% activity compared to L-tryptophan | Nostoc punctiforme | phenylpyruvate + NH3 + NADH + H+ | - |
? | |
L-phenylalanine + NAD+ + H2O | 23% activity compared to L-tryptophan | Nostoc punctiforme NIES-2108 | phenylpyruvate + NH3 + NADH + H+ | - |
? | |
L-tryptophan + NAD+ + H2O | the enzyme is highly specific for the deamination of amino acid L-tryptophan | Nostoc punctiforme | (indol-3-yl)pyruvate + NH3 + NADH + H+ | - |
r | |
L-tryptophan + NAD+ + H2O | the enzyme is highly specific for the deamination of amino acid L-tryptophan | Nostoc punctiforme NIES-2108 | (indol-3-yl)pyruvate + NH3 + NADH + H+ | - |
r | |
additional information | the enzyme shows no activity with D-tryptophan, L-tyrosine, L-alanine, L-valine, L-leucine, L-isoleucine, L-proline, L-cystine, L-methionine, L-asparagine, L-glutamine, L-aspartic acid, L-glutamic acid, L-serine, L-threonine, L-histine, L-arginine, L-lysine, glcyine, phenylpyruvate, NADP+, and NADPH | Nostoc punctiforme | ? | - |
? | |
additional information | the enzyme shows no activity with D-tryptophan, L-tyrosine, L-alanine, L-valine, L-leucine, L-isoleucine, L-proline, L-cystine, L-methionine, L-asparagine, L-glutamine, L-aspartic acid, L-glutamic acid, L-serine, L-threonine, L-histine, L-arginine, L-lysine, glcyine, phenylpyruvate, NADP+, and NADPH | Nostoc punctiforme NIES-2108 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 39500, calculated from amino acid sequence | Nostoc punctiforme |
Synonyms | Comment | Organism |
---|---|---|
NAD+-dependent L-tryptophan dehydrogenase | - |
Nostoc punctiforme |
TrpDH | - |
Nostoc punctiforme |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Nostoc punctiforme |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 55 | more than 50% activity between 35 and 55°C | Nostoc punctiforme |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | the enzyme retains full activity after incubation for 10 min at temperatures up to 40°C. There is a complete loss of activity when the enzyme is incubated at temperatures above 50°C | Nostoc punctiforme |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
L-tryptophan | at pH 11.0 and 25°C | Nostoc punctiforme | |
4.3 | - |
NAD+ | at pH 11.0 and 25°C | Nostoc punctiforme | |
16 | - |
(indol-3-yl)pyruvate | at pH 7.5 and 25°C | Nostoc punctiforme | |
17 | - |
NH3 | at pH 7.5 and 25°C | Nostoc punctiforme | |
21 | - |
NADH | at pH 7.5 and 25°C | Nostoc punctiforme |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
for amination of 3-indolpyruvate | Nostoc punctiforme |
11 | - |
for deamination of L-tryptophan | Nostoc punctiforme |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11.5 | the enzyme retains more than 80% of its activity after incubation for 30 min at pH 5.0 to 11.5 | Nostoc punctiforme |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Nostoc punctiforme | |
NADH | - |
Nostoc punctiforme |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
(indol-3-yl)pyruvate | at pH 11.0 and 25°C | Nostoc punctiforme |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
NH3 | at pH 7.5 and 25°C | Nostoc punctiforme | |
140 | - |
L-tryptophan | at pH 11.0 and 25°C | Nostoc punctiforme | |
170 | - |
NAD+ | at pH 11.0 and 25°C | Nostoc punctiforme | |
550 | - |
NADH | at pH 7.5 and 25°C | Nostoc punctiforme | |
1100 | - |
(indol-3-yl)pyruvate | at pH 7.5 and 25°C | Nostoc punctiforme |