BRENDA - Enzyme Database show
show all sequences of 1.4.1.19

L-tryptophan aminotransferase and L-tryptophan dehydrogenase, enzymes of IAA synthesis, in normal and tumorous tobacco tissue cultures

El Bahr, M.K.; Kutacek, M.; Opatrny, Z.; Biochem. Physiol. Pflanz. 182, 213-222 (1987)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.36
-
NADH
tumor C-58 cell culture
Nicotiana tabacum
1.39
-
NADH
tumor T-24 cell culture
Nicotiana tabacum
1.46
-
NADH
normal cell culture
Nicotiana tabacum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + NAD(P)+
Nicotiana tabacum
primary enzyme of indolylpyruvate pathway leading to indol-3-yl-acetic acid in plants, metabolism of indole compounds in plants
indol-3-yl-pyruvate + NH3 + NAD(P)H
-
Nicotiana tabacum
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nicotiana tabacum
-
normal and crown-gall tumor tissue cultures, tissue transformed by Agrobacterium tumefaciens
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
tissue culture
normal and crown-gall tumor tissue cultures, tissue transformed by Agrobacterium tumefaciens
Nicotiana tabacum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.87
-
cofactor NADH, activity in normal BV-N strain
Nicotiana tabacum
1.22
-
cofactor NADPH, activity in normal BV-N strain
Nicotiana tabacum
1.24
-
cofactor NADH or NADPH, activity in tumor tobacco tissue strains T-24
Nicotiana tabacum
1.57
-
cofactor NADH, activity in tumor tobacco tissue strain C-58
Nicotiana tabacum
1.59
-
cofactor NADPH, activity in tumor tobacco tissue strain C-58
Nicotiana tabacum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tryptophan + NAD(P)+
-
391536
Nicotiana tabacum
indol-3-yl-pyruvate + NH3 + NAD(P)H
-
391536
Nicotiana tabacum
r
L-tryptophan + NAD(P)+
primary enzyme of indolylpyruvate pathway leading to indol-3-yl-acetic acid in plants, metabolism of indole compounds in plants
391536
Nicotiana tabacum
indol-3-yl-pyruvate + NH3 + NAD(P)H
-
391536
Nicotiana tabacum
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Nicotiana tabacum
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)+
-
Nicotiana tabacum
NAD(P)H
normal Nicotiana tabacum cells: higher specific activity with NADPH than with NADH, tumor cells of Nicotiana tabacum: no difference in specific activity with NADPH and NADH
Nicotiana tabacum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)+
-
Nicotiana tabacum
NAD(P)H
normal Nicotiana tabacum cells: higher specific activity with NADPH than with NADH, tumor cells of Nicotiana tabacum: no difference in specific activity with NADPH and NADH
Nicotiana tabacum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.36
-
NADH
tumor C-58 cell culture
Nicotiana tabacum
1.39
-
NADH
tumor T-24 cell culture
Nicotiana tabacum
1.46
-
NADH
normal cell culture
Nicotiana tabacum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + NAD(P)+
Nicotiana tabacum
primary enzyme of indolylpyruvate pathway leading to indol-3-yl-acetic acid in plants, metabolism of indole compounds in plants
indol-3-yl-pyruvate + NH3 + NAD(P)H
-
Nicotiana tabacum
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
tissue culture
normal and crown-gall tumor tissue cultures, tissue transformed by Agrobacterium tumefaciens
Nicotiana tabacum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.87
-
cofactor NADH, activity in normal BV-N strain
Nicotiana tabacum
1.22
-
cofactor NADPH, activity in normal BV-N strain
Nicotiana tabacum
1.24
-
cofactor NADH or NADPH, activity in tumor tobacco tissue strains T-24
Nicotiana tabacum
1.57
-
cofactor NADH, activity in tumor tobacco tissue strain C-58
Nicotiana tabacum
1.59
-
cofactor NADPH, activity in tumor tobacco tissue strain C-58
Nicotiana tabacum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tryptophan + NAD(P)+
-
391536
Nicotiana tabacum
indol-3-yl-pyruvate + NH3 + NAD(P)H
-
391536
Nicotiana tabacum
r
L-tryptophan + NAD(P)+
primary enzyme of indolylpyruvate pathway leading to indol-3-yl-acetic acid in plants, metabolism of indole compounds in plants
391536
Nicotiana tabacum
indol-3-yl-pyruvate + NH3 + NAD(P)H
-
391536
Nicotiana tabacum
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Nicotiana tabacum
Other publictions for EC 1.4.1.19
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741706
Wakamatsu
Structural insights into l-tr ...
Nostoc punctiforme, Nostoc punctiforme NIES-2108
Appl. Environ. Microbiol.
83
e02710
2017
-
-
1
1
14
-
-
12
-
-
-
2
-
5
-
-
1
-
-
-
-
-
2
1
-
-
-
13
-
-
-
1
-
-
-
-
-
1
1
1
14
-
-
-
-
12
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
13
-
-
-
-
-
-
-
-
12
12
742947
Matsui
Enhancement of stability of L ...
Nostoc punctiforme, Nostoc punctiforme ATCC29133
J. Biotechnol.
196-197
27-32
2015
-
-
1
-
5
-
-
6
-
-
-
4
-
5
-
-
1
-
-
-
-
-
4
1
-
-
1
-
-
-
-
2
-
-
-
-
-
1
2
-
5
-
-
-
-
6
-
-
-
4
-
-
-
1
-
-
-
-
4
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
742410
Ogura
Biochemical characterization ...
Nostoc punctiforme, Nostoc punctiforme NIES-2108
Enzyme Microb. Technol.
60
40-46
2014
1
-
1
-
-
-
1
5
-
1
1
4
-
7
-
-
1
-
-
-
-
-
8
1
1
1
1
5
2
-
1
2
1
-
-
1
-
1
2
-
-
-
-
1
1
5
-
1
1
4
-
-
-
1
-
-
-
-
8
1
1
1
1
5
2
-
1
-
-
-
-
-
5
5
391536
El Bahr
-
L-tryptophan aminotransferase ...
Nicotiana tabacum
Biochem. Physiol. Pflanz.
182
213-222
1987
-
-
-
-
-
-
-
3
-
-
-
1
-
1
-
-
-
-
-
1
5
-
2
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
5
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
391537
Kutacek
-
Auxin biosynthesis and its reg ...
no activity in Brassica oleracea, Pisum sativum, Solanum lycopersicum, Zea mays
Biol. Plant.
27
145-153
1985
-
-
-
-
-
-
2
-
-
3
-
3
-
4
-
-
-
-
-
3
-
-
6
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
6
-
-
-
-
2
-
-
-
3
-
3
-
-
-
-
-
3
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391538
Vackova
-
Tryptophan aminotransferase an ...
Spinacia oleracea
Biol. Plant.
27
154-158
1985
-
-
-
-
-
-
-
-
3
1
-
-
-
1
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391539
Ebeid
-
Substrate specificity of L-try ...
no activity in Brassica oleracea, Pisum sativum, Prosopis juliflora, Solanum lycopersicum, Zea mays
Biol. Plant.
27
413-416
1985
-
-
-
-
-
-
-
-
-
-
-
4
-
5
-
-
-
-
-
8
10
-
8
-
4
-
-
-
-
-
-
8
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
8
10
-
8
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
391540
Tan Hoang Minh
-
Growth-correlative effect of t ...
Pisum sativum
Biol. Plant.
26
342-348
1984
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-