Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-2,6-diaminopimelate | - |
Lysinibacillus sphaericus | |
glyoxylate | 5 mM, 30% inhibition of reductive amination | Lysinibacillus sphaericus | |
HgCl2 | 0.01 mM, complete inhibition | Lysinibacillus sphaericus | |
L-2,6-diaminopimelate | - |
Lysinibacillus sphaericus | |
L-alpha,epsilon-diaminopimelate | - |
Lysinibacillus sphaericus | |
meso-alpha,delta-Diaminoadipate | - |
Lysinibacillus sphaericus | |
p-chloromercuribenzoate | 0.001 mM, complete inhibition | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00083 | - |
NADP+ | - |
Lysinibacillus sphaericus | |
0.083 | 0.14 | NADP+ | - |
Lysinibacillus sphaericus | |
0.2 | - |
NADPH | - |
Lysinibacillus sphaericus | |
0.24 | - |
L-2-amino-6-oxopimelate | - |
Lysinibacillus sphaericus | |
2.5 | - |
meso-2,6-diaminopimelate | - |
Lysinibacillus sphaericus | |
12.5 | - |
NH3 | - |
Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
2 * 41000, SDS-PAGE | Lysinibacillus sphaericus |
78500 | 80000 | gel filtration | Lysinibacillus sphaericus |
78500 | 80000 | sedimentation equilibrium centrifugation | Lysinibacillus sphaericus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-amino-6-oxoheptanedioate + NH3 + NADPH | Lysinibacillus sphaericus | - |
meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-cellulose, hydroxyapatite, Sephadex G-150 | Lysinibacillus sphaericus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
meso-2,6-diaminoheptanedioate + H2O + NADP+ = L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | catalyzes the transfer of the pro-S hydrogen at C-4 of NADPH to the substrate, enzyme is B-stereospecific | Lysinibacillus sphaericus |
Storage Stability | Organism |
---|---|
-20°C, 0.01 M potassium phosphate buffer, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, 1 year | Lysinibacillus sphaericus |
4°C, 0.01% 2-mercaptoethanol, 20% glycerol, 1 month | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H | - |
Lysinibacillus sphaericus | meso-2,6-diaminoheptanedioate + H2O + NAD(P)+ | - |
r | |
L-2-amino-6-oxoheptanedioate + NH3 + NADPH | - |
Lysinibacillus sphaericus | meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41000, SDS-PAGE | Lysinibacillus sphaericus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
unstable above | Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
2-amino-6-oxopimelate + NH3 + NADPH | Lysinibacillus sphaericus |
10.5 | - |
meso-2,6-diaminopimelate + H2O + NADP+ | Lysinibacillus sphaericus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.9 | 10 | 2-amino-6-oxopimelate + NH3 + NADPH | Lysinibacillus sphaericus |
6.8 | 11 | meso-2,6-diaminopimelate + H2O + NADP+ | Lysinibacillus sphaericus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Lysinibacillus sphaericus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.12 | - |
L-alpha,epsilon-diaminopimelate | - |
Lysinibacillus sphaericus | |
4 | - |
D-alpha,epsilon-diaminopimelate | - |
Lysinibacillus sphaericus | |
4.16 | - |
meso-alpha,delta-Diaminoadipate | - |
Lysinibacillus sphaericus |