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Literature summary for 1.4.1.16 extracted from

  • Misono, H.; Ogasawara, M.; Nagasaki, S.
    Purification and properties of meso-diaminopimelate dehydrogenase from Brevibacterium sp. (1986), Agric. Biol. Chem., 50, 1329-1330.
No PubMed abstract available
Search for more literature for 1.4.1.16

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.01 mM, 68% inhibition Brevibacterium sp.
HgCl2 0.01 mM, 100% inhibition Brevibacterium sp.
iodoacetate 1 mM, 55% inhibition Brevibacterium sp.
N-ethylmaleimide 1 mM, 52% inhibition Brevibacterium sp.
Ni2+ 1 mM, 21% inhibition Brevibacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.083 0.14 NADP+
-
 Brevibacterium sp.
0.14
-
 NADP+
-
 Brevibacterium sp.
0.21
-
 L-2-amino-6-oxopimelate
-
 Brevibacterium sp.
0.23
-
 NADPH
-
 Brevibacterium sp.
2
-
 NAD+
-
 Brevibacterium sp.
2 10 NAD+
-
 Brevibacterium sp.
6.25
-
 meso-2,6-diaminopimelate
-
 Brevibacterium sp.
62.5
-
 NH3
-
 Brevibacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 2 * 39000, SDS-PAGE Brevibacterium sp.
70000
-
 gel filtration Brevibacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-2-amino-6-oxoheptanedioate + NH3 + NADPH Brevibacterium sp.
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH Brevibacterium sp. ICR 7000
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Brevibacterium sp.
-
-
-
Brevibacterium sp. ICR 7000
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brevibacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
131
-
-
 Brevibacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Brevibacterium sp. meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Brevibacterium sp. ICR 7000 meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Brevibacterium sp. meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Brevibacterium sp. ICR 7000 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
meso-2,6-diaminoheptanedioate + H2O + NAD+
-
 Brevibacterium sp. L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
 r
meso-2,6-diaminoheptanedioate + H2O + NAD+
-
 Brevibacterium sp. ICR 7000 L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 39000, SDS-PAGE Brevibacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48
-
 10 min, 10 mM potassium phosphate pH 7.4, unstable above Brevibacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 2-amino-6-oxopimelate + NH3 + NADPH Brevibacterium sp.
10.5
-
 meso-2,6-diaminopimelate + H2O + NADP+ Brevibacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 at 40°C Brevibacterium sp.

Cofactor

Cofactor Comment Organism Structure
NADH 3% activity of that with NADP+ Brevibacterium sp.
NADPH
-
 Brevibacterium sp.