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Literature summary for 1.4.1.16 extracted from

  • Misono, H.; Ogasawara, M.; Nagasaki, S.
    Characterization of meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum and its distribution in bacteria (1986), Agric. Biol. Chem., 50, 2729-2734.
No PubMed abstract available
Search for more literature for 1.4.1.16

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Brevibacterium sp.
Cu2+ 0.01 mM, 82% inhibition Corynebacterium glutamicum
D-2,6-diaminopimelate 10 mM, 49% inhibition Corynebacterium glutamicum
D-2,6-diaminopimelate
-
 Lysinibacillus sphaericus
HgCl2
-
 Brevibacterium sp.
HgCl2 0.01 mM, 92% inhibition Corynebacterium glutamicum
HgCl2
-
 Lysinibacillus sphaericus
iodoacetate
-
 Brevibacterium sp.
iodoacetate 1 mM, 77% inhibition Corynebacterium glutamicum
iodoacetate
-
 Lysinibacillus sphaericus
L-2,6-diaminopimelate 10 mM, 27% inhibition Corynebacterium glutamicum
L-2,6-diaminopimelate
-
 Lysinibacillus sphaericus
L-cysteine 5 mM, 99% inhibition Corynebacterium glutamicum
Li+ 1 mM, 32% inhibition Corynebacterium glutamicum
N-ethylmaleimide
-
 Brevibacterium sp.
N-ethylmaleimide 1 mM, 39% inhibition Corynebacterium glutamicum
N-ethylmaleimide
-
 Lysinibacillus sphaericus
Ni2+
-
 Brevibacterium sp.
Ni2+ 1 mM, 36% inhibition Corynebacterium glutamicum
p-chloromercuribenzoate
-
 Corynebacterium glutamicum
p-chloromercuribenzoate
-
 Lysinibacillus sphaericus
thioglycolate 5 mM, 62% inhibition Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.083 0.14 NADP+
-
 Lysinibacillus sphaericus
0.083 0.14 NADP+
-
 Corynebacterium glutamicum
0.083 0.14 NADP+
-
 Brevibacterium sp.
0.13
-
 NADPH
-
 Corynebacterium glutamicum
0.28
-
 L-2-amino-6-oxopimelate
-
 Corynebacterium glutamicum
2 10 NAD+
-
 Corynebacterium glutamicum
2 10 NAD+
-
 Brevibacterium sp.
10
-
 NAD+
-
 Corynebacterium glutamicum
36
-
 NH3
-
 Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 2 * 39000, SDS-PAGE Corynebacterium glutamicum
70000
-
-
 Brevibacterium sp.
70000
-
 gel filtration Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-2-amino-6-oxoheptanedioate + NH3 + NADPH Lysinibacillus sphaericus
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH Corynebacterium glutamicum
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH Pseudomonas fragi
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH Brevibacterium sp.
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH Morganella morganii
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH Providencia rettgeri
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH Pseudomonas cruciviae
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH Glutamicibacter protophormiae
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Achromobacter polymorph
-
 very low activity
-
Achromobacter superficialis
-
 very low activity
-
Agrobacterium tumefaciens
-
 very low activity
-
Alcaligenes viscolactis
-
 low activity
-
Bacillus amyloliquefaciens
-
 very low activity
-
Bacillus cereus
-
 very low activity
-
Bacillus licheniformis
-
 very low activity
-
Bacterium mycoides
-
 very low activity
-
Brevibacillus brevis
-
 low activity
-
Brevibacterium sp.
-
-
-
Corynebacterium ammoniagenes
-
 low activity
-
Corynebacterium glutamicum
-
 strain ATCC 13032
-
Corynebacterium pseudodiphtheriticum
-
 low activity
-
Enterobacter cloacae
-
 low activity
-
Flavobacterium suaveolens
-
 low activity
-
Glutamicibacter protophormiae
-
-
-
Hafnia alvei
-
 very low activity
-
Kocuria rosea
-
 very low activity
-
Lysinibacillus sphaericus
-
-
-
Micrococcus luteus
-
 low activity
-
Morganella morganii
-
-
-
no activity in Escherichia coli
-
 strains ICR 0010, K12, ICR 0050 and IFO 3301
-
no activity in Klebsiella pneumoniae
-
 strains IFO 125059 and 12019
-
no activity in Staphylococcus aureus
-
-
-
Providencia rettgeri
-
-
-
Pseudomonas aeruginosa
-
 very low activity
-
Pseudomonas alkanolytica
-
 very low activity
-
Pseudomonas chlororaphis subsp. aureofaciens
-
 very low activity
-
Pseudomonas cruciviae
-
-
-
Pseudomonas fragi
-
-
-
Pseudomonas putida
-
 very low activity
-
Terrabacter tumescens
-
 very low activity
-

Purification (Commentary)

Purification (Comment) Organism
-
 Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
131.6
-
-
 Corynebacterium glutamicum

Storage Stability

Storage Stability Organism
-20°C, 0.01 M potassium phosphate buffer, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, 1 year Lysinibacillus sphaericus
-20°C, 0.01 M potassium phosphate buffer, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, 1 year Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Brevibacillus brevis meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Enterobacter cloacae meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Lysinibacillus sphaericus meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Kocuria rosea meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Pseudomonas aeruginosa meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Pseudomonas putida meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Bacillus cereus meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Corynebacterium glutamicum meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Micrococcus luteus meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Bacillus licheniformis meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Bacillus amyloliquefaciens meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Pseudomonas fragi meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Agrobacterium tumefaciens meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Brevibacterium sp. meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Morganella morganii meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Hafnia alvei meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Corynebacterium ammoniagenes meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Providencia rettgeri meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Pseudomonas cruciviae meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Pseudomonas chlororaphis subsp. aureofaciens meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Glutamicibacter protophormiae meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Corynebacterium pseudodiphtheriticum meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Alcaligenes viscolactis meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Achromobacter polymorph meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Pseudomonas alkanolytica meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Flavobacterium suaveolens meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Terrabacter tumescens meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Bacterium mycoides meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Achromobacter superficialis meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Lysinibacillus sphaericus meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Corynebacterium glutamicum meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Pseudomonas fragi meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Brevibacterium sp. meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Morganella morganii meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Providencia rettgeri meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Pseudomonas cruciviae meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Glutamicibacter protophormiae meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
meso-2,6-diaminoheptanedioate + H2O + NAD+
-
 Corynebacterium glutamicum L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
 r
meso-2,6-diaminoheptanedioate + H2O + NAD+
-
 Brevibacterium sp. L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
 r

Subunits

Subunits Comment Organism
dimer
-
 Lysinibacillus sphaericus
dimer
-
 Brevibacterium sp.
dimer 2 * 39000, SDS-PAGE Corynebacterium glutamicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 unstable above Lysinibacillus sphaericus
45
-
 unstable above Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 reductive amination of 2-amino-6-oxopimelate Corynebacterium glutamicum
9
-
 oxidative deamination of meso-2,6-diaminopimelate Corynebacterium glutamicum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 7
-
 Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
NADH 3% activity of that with NADP+ Corynebacterium glutamicum
NADH 3% activity of that with NADP+ Brevibacterium sp.
NADPH
-
 Brevibacillus brevis
NADPH
-
 Enterobacter cloacae
NADPH
-
 Lysinibacillus sphaericus
NADPH
-
 Kocuria rosea
NADPH
-
 Pseudomonas aeruginosa
NADPH
-
 Pseudomonas putida
NADPH
-
 Bacillus cereus
NADPH
-
 Corynebacterium glutamicum
NADPH
-
 Micrococcus luteus
NADPH
-
 Bacillus licheniformis
NADPH
-
 Bacillus amyloliquefaciens
NADPH
-
 Pseudomonas fragi
NADPH
-
 Agrobacterium tumefaciens
NADPH
-
 Brevibacterium sp.
NADPH
-
 Morganella morganii
NADPH
-
 Hafnia alvei
NADPH
-
 Corynebacterium ammoniagenes
NADPH
-
 Providencia rettgeri
NADPH
-
 Pseudomonas cruciviae
NADPH
-
 Pseudomonas chlororaphis subsp. aureofaciens
NADPH
-
 Glutamicibacter protophormiae
NADPH
-
 Corynebacterium pseudodiphtheriticum
NADPH
-
 Alcaligenes viscolactis
NADPH
-
 Achromobacter polymorph
NADPH
-
 Pseudomonas alkanolytica
NADPH
-
 Flavobacterium suaveolens
NADPH
-
 Terrabacter tumescens
NADPH
-
 Bacterium mycoides
NADPH
-
 Achromobacter superficialis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2
-
 D-2,6-diaminopimelate
-
 Corynebacterium glutamicum
8.5
-
 L-2,6-diaminopimelate
-
 Corynebacterium glutamicum