Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-glutamate | competitive with 2-oxoglutarate; glutamate binds 1.7times more tightly in the presence of NAD+ | Lupinus angustifolius | |
NAD+ | competitive with NADH | Lupinus angustifolius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00124 | - |
NADH | - |
Lupinus angustifolius | |
0.0238 | - |
2-oxoglutarate | - |
Lupinus angustifolius | |
0.5 | - |
L-glutamine | - |
Lupinus angustifolius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lupinus angustifolius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lupinus angustifolius |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 L-glutamate + NAD+ = L-glutamine + 2-oxoglutarate + NADH + H+ | kinetic mechanism | Lupinus angustifolius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root nodule | - |
Lupinus angustifolius | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADH | 1 mol 2-oxoglutarate and 1 mol glutamine are consumed per mol NADH oxidized | Lupinus angustifolius | L-glutamate + NAD+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADH | mechanism involves compulsory, very tightly binding of NADH as first substrate, followed by random-order binding of glutamine and 2-oxoglutarate, NADH triggers a conformational change, which then exposes the binding sites of the other substrates | Lupinus angustifolius | L-glutamate + NAD+ | - |
? | |
additional information | sodium dithionite is no alternative reductant | Lupinus angustifolius | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Lupinus angustifolius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavoprotein | Lupinus angustifolius | |
NADH | - |
Lupinus angustifolius |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
glutamate | - |
Lupinus angustifolius |