Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.14 extracted from

  • Boland, M.J.
    Kinetic mechanism of NADH-dependent glutamate synthase from lupin nodules (1979), Eur. J. Biochem., 99, 531-539.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
L-glutamate competitive with 2-oxoglutarate; glutamate binds 1.7times more tightly in the presence of NAD+ Lupinus angustifolius
NAD+ competitive with NADH Lupinus angustifolius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00124
-
NADH
-
Lupinus angustifolius
0.0238
-
2-oxoglutarate
-
Lupinus angustifolius
0.5
-
L-glutamine
-
Lupinus angustifolius

Organism

Organism UniProt Comment Textmining
Lupinus angustifolius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lupinus angustifolius

Reaction

Reaction Comment Organism Reaction ID
2 L-glutamate + NAD+ = L-glutamine + 2-oxoglutarate + NADH + H+ kinetic mechanism Lupinus angustifolius

Source Tissue

Source Tissue Comment Organism Textmining
root nodule
-
Lupinus angustifolius
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH 1 mol 2-oxoglutarate and 1 mol glutamine are consumed per mol NADH oxidized Lupinus angustifolius L-glutamate + NAD+
-
?
L-glutamine + 2-oxoglutarate + NADH mechanism involves compulsory, very tightly binding of NADH as first substrate, followed by random-order binding of glutamine and 2-oxoglutarate, NADH triggers a conformational change, which then exposes the binding sites of the other substrates Lupinus angustifolius L-glutamate + NAD+
-
?
additional information sodium dithionite is no alternative reductant Lupinus angustifolius ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Lupinus angustifolius

Cofactor

Cofactor Comment Organism Structure
flavin flavoprotein Lupinus angustifolius
NADH
-
Lupinus angustifolius

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1
-
glutamate
-
Lupinus angustifolius