Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.13 extracted from

  • Jongsareejit, B.; Rahman, R.N.Z.A.; Fujiwara, S.; Imanaka, T.
    Gene cloning, sequencing and enzymic properties of glutamate synthase from the hyperthermophilic archaeon Pyrococcus sp. KOD1 (1997), Mol. Gen. Genet., 254, 635-642.
    View publication on PubMed
Search for more literature for 1.4.1.13

Cloned(Commentary)

Cloned (Comment) Organism
the gltA gene encoding the enzyme overexpressed in Escherichia coli Pyrococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 4 * 53000, SDS-PAGE Pyrococcus sp.
205000
-
 gel filtration Pyrococcus sp.

Organism

Organism UniProt Comment Textmining
Pyrococcus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using heat treatment, anion-exchange column chromatography on HiTrap Q and cation exchange column chromatography on Resources S Pyrococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1910
-
-
 Pyrococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADPH + H+
-
 Pyrococcus sp. L-glutamate + NADP+
-
 ?
NH3 + 2-oxoglutarate + NADPH + H+
-
 Pyrococcus sp. L-glutamate + NADP+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 53000, SDS-PAGE Pyrococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 glutamine-dependent reaction Pyrococcus sp.
90
-
 NH3-dependent reaction Pyrococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 optimum for both NH3-dependent and L-glutamine-dependent reaction Pyrococcus sp.

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Pyrococcus sp.