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Literature summary for 1.4.1.13 extracted from

  • Rachim, M.A.; Nicholas, D.J.D.
    Glutamine synthetase and glutamate synthase from Sclerotinia Schlerotiorum (1985), Phytochemistry, 24, 2541-2548.
No PubMed abstract available
Search for more literature for 1.4.1.13

Inhibitors

Inhibitors Comment Organism Structure
2,2'-bipyridyl 88% inhibition at 10 mM Sclerotinia sclerotiorum
ADP
-
 Sclerotinia sclerotiorum
AMP
-
 Sclerotinia sclerotiorum
arginine less than 45% inhibition Sclerotinia sclerotiorum
ATP restricts activity by 47% at 5 mM Sclerotinia sclerotiorum
azaserine 68% inhibition at 1 mM Sclerotinia sclerotiorum
citrate slight Sclerotinia sclerotiorum
D-glutamate
-
 Sclerotinia sclerotiorum
DL-methionine DL-sulfoximine
-
 Sclerotinia sclerotiorum
L-leucine less than 45% inhibition Sclerotinia sclerotiorum
L-malate 40% inhibition at 5 mM Sclerotinia sclerotiorum
o-phenanthroline complete inhibition at 10 mM Sclerotinia sclerotiorum
oxalate 48% inhibition at 5 mM Sclerotinia sclerotiorum
oxaloacetate
-
 Sclerotinia sclerotiorum
p-chloromercuribenzoate complete inhibition at 0.1 mM Sclerotinia sclerotiorum
phenylalanine 78% inhibition at 10 mM Sclerotinia sclerotiorum
pyruvate
-
 Sclerotinia sclerotiorum
sodium arsenite 57% inhibition at 10 mM Sclerotinia sclerotiorum
succinate
-
 Sclerotinia sclerotiorum
valine less than 45% inhibition Sclerotinia sclerotiorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 NADPH
-
 Sclerotinia sclerotiorum
0.35
-
 alpha-ketoglutarate
-
 Sclerotinia sclerotiorum
2.6
-
 L-glutamine
-
 Sclerotinia sclerotiorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADPH Sclerotinia sclerotiorum main route for assimilation of ammonium compounds L-glutamate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Sclerotinia sclerotiorum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation, column chromatography on DEAE-cellulose and Blue-Sepharose Sclerotinia sclerotiorum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.6
-
 partially purified enzyme Sclerotinia sclerotiorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADPH main route for assimilation of ammonium compounds Sclerotinia sclerotiorum L-glutamate + NADP+
-
 ?
L-glutamine + 2-oxoglutarate + NADPH + H+
-
 Sclerotinia sclerotiorum L-glutamate + NADP+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADH no activity with NADH Sclerotinia sclerotiorum
NADPH
-
 Sclerotinia sclerotiorum