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Literature summary for 1.4.1.13 extracted from
- Glutamate synthase. Properties of the glutamine-dependent activity (1976), J. Biol. Chem., 251, 3294-3299.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-Bromo-2-oxoglutarate | - |
Escherichia coli |  |
| 4-Iodoacetamidosalicylate | - |
Escherichia coli | |
| Bromopyruvate | - |
Escherichia coli | |
| iodoacetamide | selective inactivation of glutamine-dependent activity | Escherichia coli | |
| L-2-Amino-4-oxo-5-chloropentanoic acid | selective inhibitor of glutamine-dependent activity | Escherichia coli | |
| N-ethylmaleimide | - |
Escherichia coli | |
| NADPH | inactivation of glutamine-dependent activity, 50% inactivation at 0.36 mM in 10 min | Escherichia coli | |
| p-chloromercuribenzoate | - |
Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using streptomycin sulfate treatment, ammonium sulfate fractionation, heat treatment, agarose gel filtration and DEAE-cellulose column chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.3 | - |
native enzyme | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-oxoglutarate + NADPH + H+ | in the reverse reaction ammonia can act instead of glutamine, but more slowly | Escherichia coli | L-glutamate + NADP+ | - |
? | |
| NH3 + 2-oxoglutarate + NADPH + H+ | the specific activity of native enzyme using NH3 varies between 5% and 7% of the glutamine-dependent activity | Escherichia coli | L-glutamate + NADP+ | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
L-2-Amino-4-oxo-5-chloropentanoic acid | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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