General Stability | Organism |
---|---|
2-mercaptoethanol, stabilizes | Thiobacillus thioparus |
DTT, 1 mM, stabilizes at 4°C | Thiobacillus thioparus |
EDTA, stabilizes | Thiobacillus thioparus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-bipyridyl | 100% inhibition at 3 mM | Thiobacillus thioparus | |
Atebrin | 100% inhibition at 1 mM | Thiobacillus thioparus | |
ATP | 33% inhibition at 20 mM | Thiobacillus thioparus | |
D-glutamate | - |
Thiobacillus thioparus | |
iodoacetate | 27% inhibition at 25 mM | Thiobacillus thioparus | |
isocitrate | 32% inhibition at 20 mM | Thiobacillus thioparus | |
KCN | 89% inhibition at 20 mM | Thiobacillus thioparus | |
L-glutamate | more than 40% inhibition at 50 mM | Thiobacillus thioparus | |
L-histidine | more than 40% inhibition at 50 mM | Thiobacillus thioparus | |
L-methionine | more than 40% inhibition at 50 mM | Thiobacillus thioparus | |
L-serine | more than 40% inhibition at 10 mM and 50 mM | Thiobacillus thioparus | |
additional information | a combination of L-serine, L-methionine, L-alanine, L-glycine, L-histidine and L-asparagine inhibits 73% of the acitivity at 7.5 mM each | Thiobacillus thioparus | |
N-ethylmaleimide | 83% inhibition at 10 mM | Thiobacillus thioparus | |
NADP+ | 59% inhibition at 1 mM | Thiobacillus thioparus | |
NaN3 | 100% inhibition at 50 mM, 11% inhibition at 20 mM | Thiobacillus thioparus | |
o-phenanthroline | 37% inhibition at 10 mM | Thiobacillus thioparus | |
oxaloacetate | more than 50% inhibition at 20 mM | Thiobacillus thioparus | |
p-hydroxymercuribenzoate | complete inhibition at 1 mM, 5 min | Thiobacillus thioparus | |
sodium arsenite | 100% inhibition at 50 mM , 10% inhibition at 20 mM | Thiobacillus thioparus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
NADPH | - |
Thiobacillus thioparus | |
0.05 | - |
alpha-ketoglutarate | - |
Thiobacillus thioparus | |
1.1 | - |
L-glutamine | - |
Thiobacillus thioparus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
x * 72000 + x * 94000 | Thiobacillus thioparus |
94000 | - |
x * 72000 + x * 94000 | Thiobacillus thioparus |
280000 | - |
gel filtration | Thiobacillus thioparus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thiobacillus thioparus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate fractionation, column chromatography on DEAE-cellulose and Sephadex G-200 | Thiobacillus thioparus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thiobacillus thioparus |
9.3 | - |
- |
Thiobacillus thioparus |
Storage Stability | Organism |
---|---|
storing at -20°C, total loss of activity after 10 days | Thiobacillus thioparus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADPH + H+ | in the reverse reaction ammonia can act instead of glutamine, but more slowly | Thiobacillus thioparus | L-glutamate + NADP+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADPH + H+ | NH3-dependent activity is increased approximately 5-fold in apoglutamate synthase lacking flavin and non-heme iron | Thiobacillus thioparus | L-glutamate + NADP+ | - |
? | |
additional information | alpha-ketoglutarate can not be replaced with pyruvate or oxalacetate | Thiobacillus thioparus | ? | - |
? | |
NH3 + 2-oxoglutarate + NADPH + H+ | ammonia activity with 100 mM NH4Cl is about 6% of the glutamine activity with 5 mM L-glutamine | Thiobacillus thioparus | L-glutamate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000 + x * 94000 | Thiobacillus thioparus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-20 | - |
complete loss of activity after 10 days | Thiobacillus thioparus |
4 | - |
unprotected enzyme loses 90% of activity in 48 hours, protected enzyme, addition of 5 mM EDTA and 5 mM 2-mercaptoethanol or 1 mM DTT retains 80 to 90% of the activity after 48 hours | Thiobacillus thioparus |
100 | - |
30 s, complete loss of activity | Thiobacillus thioparus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | 7.8 | - |
Thiobacillus thioparus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavoenzyme | Thiobacillus thioparus | |
NADH | no activity with NADH | Thiobacillus thioparus | |
NADPH | - |
Thiobacillus thioparus |