Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dimethyl suberimidate | increases NH3-dependent activity | Priestia megaterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dimethyl suberimidate | inactivates glutamine-dependent activity | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0071 | - |
NADPH | - |
Priestia megaterium | |
0.009 | - |
2-oxoglutarate | - |
Priestia megaterium | |
0.2 | - |
L-glutamine | - |
Priestia megaterium | |
22 | - |
NH4Cl | - |
Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 142000 + x * 55000, SDS-PAGE | Priestia megaterium |
142000 | - |
x * 142000 + x * 55000, SDS-PAGE | Priestia megaterium |
840000 | - |
sucrose density-gradient centrifugation | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using streptomycin sulfate treatment, ammonium sulfate precipitation, column chromatography on Ultrogel AcA 22 and DEAE-Sephadex A-50, ultrafiltration with an Amicon PM 30 membrane and chromatography on hydroxyapatite column | Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23.8 | - |
- |
Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADPH + H+ | in the reverse reaction ammonia can act instead of glutamine, but more slowly | Priestia megaterium | L-glutamate + NADP+ | - |
? | |
NH3 + 2-oxoglutarate + NADPH + H+ | 2% to 4% relative activity to L-glutamine | Priestia megaterium | L-glutamate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 142000 + x * 55000, SDS-PAGE | Priestia megaterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Priestia megaterium |