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Literature summary for 1.4.1.13 extracted from
- Nitrogen metabolism in Rhodospirillum rubrum: Characterization of glutamate synthase (1981), J. Gen. Microbiol., 123, 335-342.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
NADPH | - |
Rhodospirillum rubrum |  |
| 0.035 | - |
2-oxoglutarate | - |
Rhodospirillum rubrum | |
| 0.13 | - |
L-glutamine | - |
Rhodospirillum rubrum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| thylakoid membrane | association with | Rhodospirillum rubrum | 42651 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | iron-sulfur protein | Rhodospirillum rubrum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
MW is salt-dependent | Rhodospirillum rubrum |
| 260000 | - |
gel filtration, in the absence of salt | Rhodospirillum rubrum |
| 840000 | - |
gel filtration, in the presence of 0.4 M NaCl | Rhodospirillum rubrum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-oxoglutarate + NADPH | Rhodospirillum rubrum | glutamate biosynthesis | L-glutamate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodospirillum rubrum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, using ultrasonic oscillation, ammonium sulfate precipitation, column chromatography on Sephacryl S300 and DE-52 cellulose | Rhodospirillum rubrum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rhodospirillum rubrum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-oxoglutarate + NADPH | glutamate biosynthesis | Rhodospirillum rubrum | L-glutamate + NADP+ | - |
? | |
| L-glutamine + 2-oxoglutarate + NADPH + H+ | - |
Rhodospirillum rubrum | L-glutamate + NADP+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 43 | - |
- |
Rhodospirillum rubrum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| -10 | - |
stable for at least 6 weeks | Rhodospirillum rubrum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Rhodospirillum rubrum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | flavoenzyme | Rhodospirillum rubrum | |
| NADH | no activity with NADH | Rhodospirillum rubrum | |
| NADPH | - |
Rhodospirillum rubrum | |
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Release 2023.1 (January 2023)
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