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Literature summary for 1.4.1.13 extracted from

  • Schmidt, C.N.G.; Jervis, L.
    Partial purification and characterization of glutamate synthase from a thermophilic bacillus (1982), J. Gen. Microbiol., 128, 1713-1718.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
azaserine in both the L-glutamine- and NH4Cl-dependent reactions Geobacillus stearothermophilus
Cibacron blue 3GA
-
Geobacillus stearothermophilus
L-methionine sulfone
-
Geobacillus stearothermophilus
NADPH rapid inactivation in absence of L-glutamine and 2-oxoglutarate, capable of causing 50% inactivation in 5 min at a concentration of 1 mM Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
2-oxoglutarate
-
Geobacillus stearothermophilus
0.022
-
NADPH
-
Geobacillus stearothermophilus
0.029
-
L-glutamine
-
Geobacillus stearothermophilus
44
-
NH4Cl
-
Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
gel filtration Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation, gel filtration on Sephacryl S-300 and affinity chromatography on NADPH-Sepharose Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADPH + H+ in the reverse reaction ammonia can act instead of glutamine, but more slowly Geobacillus stearothermophilus L-glutamate + NADP+
-
ir
NH3 + 2-oxoglutarate + NADPH + H+ the rate is only 10% to 15% that of the L-glutamine-dependent reaction Geobacillus stearothermophilus L-glutamate + NADP+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
glutamine-dependent reaction Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
10 min, 35% loss of activity Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
glutamine-dependent activity Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
NADH no activity with NADH Geobacillus stearothermophilus
NADPH
-
Geobacillus stearothermophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.012
-
Cibacron blue 3GA
-
Geobacillus stearothermophilus
0.022
-
L-methionine sulfone
-
Geobacillus stearothermophilus