Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azaserine | in both the L-glutamine- and NH4Cl-dependent reactions | Geobacillus stearothermophilus | |
Cibacron blue 3GA | - |
Geobacillus stearothermophilus | |
L-methionine sulfone | - |
Geobacillus stearothermophilus | |
NADPH | rapid inactivation in absence of L-glutamine and 2-oxoglutarate, capable of causing 50% inactivation in 5 min at a concentration of 1 mM | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
2-oxoglutarate | - |
Geobacillus stearothermophilus | |
0.022 | - |
NADPH | - |
Geobacillus stearothermophilus | |
0.029 | - |
L-glutamine | - |
Geobacillus stearothermophilus | |
44 | - |
NH4Cl | - |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate fractionation, gel filtration on Sephacryl S-300 and affinity chromatography on NADPH-Sepharose | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADPH + H+ | in the reverse reaction ammonia can act instead of glutamine, but more slowly | Geobacillus stearothermophilus | L-glutamate + NADP+ | - |
ir | |
NH3 + 2-oxoglutarate + NADPH + H+ | the rate is only 10% to 15% that of the L-glutamine-dependent reaction | Geobacillus stearothermophilus | L-glutamate + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
glutamine-dependent reaction | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
10 min, 35% loss of activity | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
glutamine-dependent activity | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | no activity with NADH | Geobacillus stearothermophilus | |
NADPH | - |
Geobacillus stearothermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
Cibacron blue 3GA | - |
Geobacillus stearothermophilus | |
0.022 | - |
L-methionine sulfone | - |
Geobacillus stearothermophilus |