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Literature summary for 1.4.1.13 extracted from

  • Sung, H.C.; Tachiki, T.; Kumagai, H.; Tochikura, T.
    Properties of glutamate synthase from Brevibachterium flavum (1984), J. Ferment. Technol., 62, 569-575.
No PubMed abstract available
Search for more literature for 1.4.1.13

General Stability

General Stability Organism
alpha-ketoglutarate stabilizes [Brevibacterium] flavum
glutamine stabilizes [Brevibacterium] flavum

Inhibitors

Inhibitors Comment Organism Structure
2'-adenylic acid
-
 [Brevibacterium] flavum
AgNO3 at 0.001 mM completely inhibits [Brevibacterium] flavum
ATP at 3.5 mM 12% inhibition [Brevibacterium] flavum
D-glutamate at 3.5 mM 10% inhibition [Brevibacterium] flavum
D-Lysine significantly inhibits [Brevibacterium] flavum
HgCl2 at 0.001 mM remains 8% of activity [Brevibacterium] flavum
iodoacetate completely inhibits at 1 mM, 2-mercaptoethanol protects against inhibition [Brevibacterium] flavum
L-glutamate at 3.5 mM 13% inhibition [Brevibacterium] flavum
L-histidine significant inhibitor [Brevibacterium] flavum
L-homoserine significant inhibitor [Brevibacterium] flavum
L-malate at 3.5 mM 67% inhibition [Brevibacterium] flavum
L-methionine
-
 [Brevibacterium] flavum
lysine significantly inhibits [Brevibacterium] flavum
methionine sulfone
-
 [Brevibacterium] flavum
NADH at 3.5 mM 31% inhibition [Brevibacterium] flavum
NADP+ 76% inhibition [Brevibacterium] flavum
oxalglycine
-
 [Brevibacterium] flavum
p-chloromercuribenzoate completely inhibits at 1 mM, 2-mercaptoethanol protects against inhibition [Brevibacterium] flavum
phenylalanine significantly inhibits [Brevibacterium] flavum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 NADPH
-
 [Brevibacterium] flavum
0.06
-
 alpha-ketoglutarate
-
 [Brevibacterium] flavum
0.24
-
 L-glutamine
-
 [Brevibacterium] flavum

Metals/Ions

Metals/Ions Comment Organism Structure
Iron enzyme contains 8 mol of non-heme iron [Brevibacterium] flavum

Organism

Organism UniProt Comment Textmining
[Brevibacterium] flavum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation, column chromatography on DEAE-cellulose, Sepharose 6B, hydroxyapatite, glutamate dehydrogenase antibody affinity chromatography and column chromatography on Sephacryl S-200 [Brevibacterium] flavum

Reaction

Reaction Comment Organism Reaction ID
2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+ hexa-uni ping pong mechanism [Brevibacterium] flavum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30.5
-
-
 [Brevibacterium] flavum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADPH + H+ highly specific [Brevibacterium] flavum L-glutamate + NADP+
-
 ?
L-glutamine + 2-oxoglutarate + NADPH + H+ glyoxylate shows 3% reactivity compared with alpha-ketoglutarate [Brevibacterium] flavum L-glutamate + NADP+
-
 ?
L-glutamine + 2-oxoglutarate + NADPH + H+ ammonia does not replace L-glutamine as amino donor [Brevibacterium] flavum L-glutamate + NADP+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 [Brevibacterium] flavum

Cofactor

Cofactor Comment Organism Structure
flavin enzyme contains 2 mol of FMN and 2 mol of FAD [Brevibacterium] flavum
NADH no activity with NADH [Brevibacterium] flavum
NADPH
-
 [Brevibacterium] flavum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
 methionine sulfone competitive inhibitor with respect to glutamine [Brevibacterium] flavum
0.041
-
 methionine sulfone uncompetitive inhibitor with respect to alpha-ketoglutarate [Brevibacterium] flavum
0.045
-
 methionine sulfone uncompetitive inhibitor with respect to NADPH [Brevibacterium] flavum
0.25
-
 NADP competitive inhibitor vs. NADPH [Brevibacterium] flavum
0.69
-
 NADP uncompetitive inhibitor vs. alpha-ketoglutarate [Brevibacterium] flavum
0.8
-
 oxalylglycine competitive inhibitor with respect to alpha-ketoglutarate [Brevibacterium] flavum
1.13
-
 NADP uncompetitive inhibitor vs. glutamine [Brevibacterium] flavum
2.1
-
 2'-adenylic acid competitive inhibitor with respect to NADPH [Brevibacterium] flavum
17.1
-
 2'-adenylic acid uncompetitive inhibitor with respect to glutamine [Brevibacterium] flavum
17.2
-
 2'-adenylic acid uncompetitive inhibitor with respect to alpha-keto-glutarate [Brevibacterium] flavum
19.3
-
 oxalylglycine uncompetitive inhibitor with respect to glutamine [Brevibacterium] flavum
20.6
-
 oxalylglycine uncompetitive inhibitor with respect to NADP [Brevibacterium] flavum
25
-
 glutamate competitive inhibitor with respect to glutamine [Brevibacterium] flavum
45
-
 glutamate competitive inhibitor with respect to alpha-ketoglutarate [Brevibacterium] flavum