Application | Comment | Organism |
---|---|---|
synthesis | the enzyme can be used as heterogeneous biocatalyst for reductive aminations of 2-oxoacids | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the purified enzyme is immobilized on porous agarose microbeads activated with glyoxyl groups (aliphatic aldehydes). 85% of the offered enzyme is immobilized on these microbeads and the enzyme recovers 45% of its initial reduction amination activity upon the multivalent and irreversible attachment. The immobilized enzyme shows 13fold increased thermostability compared to the soluble enzyme. The optimally immobilized enzyme is also stabilized against acidic pH. The enzyme works as heterogeneous biocatalyst for the synthesis of L-[13N]alanine using pyruvate and [13N]NH4OH obtaining a radiochemical yield of over 95% in 20 minutes. This immobilized enzyme is reused for up to 5 cycles keeping the maximum yield. Immobilization method optimization, overview | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + H2O + NAD+ | Bacillus subtilis | - |
pyruvate + NH3 + NADH + H+ | - |
r | |
L-alanine + H2O + NAD+ | Bacillus subtilis 168 | - |
pyruvate + NH3 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | Q08352 | - |
- |
Bacillus subtilis 168 | Q08352 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + H2O + NAD+ | - |
Bacillus subtilis | pyruvate + NH3 + NADH + H+ | - |
r | |
L-alanine + H2O + NAD+ | - |
Bacillus subtilis 168 | pyruvate + NH3 + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
L-AlaDH | - |
Bacillus subtilis |
L-AlaDH-Bs | - |
Bacillus subtilis |
L-alanine dehydrogenase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | assay at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis | |
NADH | - |
Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
physiological function | L-alanine dehydrogenase is a NADH-dependent enzyme that catalyzes the reversible reductive amination of pyruvate using ammonia as amine source | Bacillus subtilis |