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Literature summary for 1.4.1.1 extracted from

  • Irwin, J.A.; Lynch, S.V.; Coughlan, S.; Baker, P.J.; Gudmundsson, H.M.; Alfredsson, G.A.; Rice, D.W.; Engel, P.C.
    Alanine dehydrogenase from the psychrophilic bacterium strain PA-43: overexpression, molecular characterization, and sequence analysis (2003), Extremophiles, 7, 135-143.
    View publication on PubMed
Search for more literature for 1.4.1.1

Activating Compound

Activating Compound Comment Organism Structure
beta-mercaptoethanol slight activatory effect Shewanella sp.

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Shewanella sp.

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 1 mM, 15% inhibition Shewanella sp.
Cibacron blue F3GA 0.1 mM, 70% inhibition Shewanella sp.
DTNB 7% inhibition with 0.01 M and 40% inhibition with 0.1 mM Shewanella sp.
L-Cys 10 mM, 40% inhibition Shewanella sp.
MnCl2 1 mM, 10% inhibition Shewanella sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 NAD+ pH 10.0, 10°C or 15°C Shewanella sp.
0.04
-
 NADH pH 10.0, 5°C Shewanella sp.
0.05
-
 NAD+ pH 10.0, 25°C Shewanella sp.
0.07
-
 NAD+ pH 10.0, 35°C Shewanella sp.
0.08
-
 NADH pH 10.0, 25°C or 40°C Shewanella sp.
0.1
-
 NAD+ pH 10.0, 45°C Shewanella sp.
0.16
-
 NAD+ pH 10.0, 50°C Shewanella sp.
0.23
-
 NAD+ pH 10.0, 55°C Shewanella sp.
0.39
-
 pyruvate pH 10.0, 5°C Shewanella sp.
0.8
-
 pyruvate pH 10.0, 25°C Shewanella sp.
0.8
-
 pyruvate pH 10.0, 40°C Shewanella sp.
2 3 L-Ala pH 10.0, 55°C Shewanella sp.
9.7
-
 L-Ala pH 10.0, 25°C Shewanella sp.
10.1
-
 L-Ala pH 10.0, 10°C Shewanella sp.
10.6
-
 L-Ala pH 10.0, 15°C Shewanella sp.
10.8
-
 L-Ala pH 10.0, 45°C Shewanella sp.
25
-
 NH4+ pH 10.0, 25°C Shewanella sp.
28
-
 NH4+ pH 10.0, 5°C Shewanella sp.
30
-
 NH4+ pH 10.0, 40°C Shewanella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39596
-
 x * 39596, calculation from nucleotide sequence Shewanella sp.
260000
-
 gel filtration Shewanella sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Ala + H2O + NAD+ Shewanella sp.
-
 pyruvate + NH3 + NADH
-
 ?
L-Ala + H2O + NAD+ Shewanella sp. PA-43
-
 pyruvate + NH3 + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Shewanella sp. Q8RLH6
-
-
Shewanella sp. PA-43 Q8RLH6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Shewanella sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.8
-
-
 Shewanella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala + H2O + NAD+
-
 Shewanella sp. pyruvate + NH3 + NADH
-
 ?
L-Ala + H2O + NAD+
-
 Shewanella sp. PA-43 pyruvate + NH3 + NADH
-
 ?

Subunits

Subunits Comment Organism
? x * 39596, calculation from nucleotide sequence Shewanella sp.

Synonyms

Synonyms Comment Organism
ALADH
-
 Shewanella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52
-
 half-life: 9 min Shewanella sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19
-
 NAD+ pH 10.0, 10°C Shewanella sp.
41
-
 NAD+ pH 10.0, 15°C Shewanella sp.
45
-
 L-Ala pH 10.0, 10°C Shewanella sp.
63
-
 NAD+ pH 10.0, 25°C Shewanella sp.
67
-
 L-Ala pH 10.0, 15°C Shewanella sp.
75
-
 L-Ala pH 10.0, 25°C Shewanella sp.
128
-
 NAD+ pH 10.0, 35°C Shewanella sp.
163
-
 NAD+ pH 10.0, 55°C Shewanella sp.
207
-
 NAD+ pH 10.0, 45°C Shewanella sp.
211
-
 NAD+ pH 10.0, 50°C Shewanella sp.
215
-
 L-Ala pH 10.0, 55°C Shewanella sp.
228
-
 L-Ala pH 10.0, 45°C Shewanella sp.
888
-
 pyruvate pH 10.0, 5°C Shewanella sp.
977
-
 NH4+ pH 10.0, 5°C Shewanella sp.
1008
-
 NADH pH 10.0, 5°C Shewanella sp.
1755
-
 NH4+ pH 10.0, 25°C Shewanella sp.
1963
-
 pyruvate pH 10.0, 25°C Shewanella sp.
2209
-
 NADH pH 10.0, 25°C Shewanella sp.
2472
-
 NH4+ pH 10.0, 40°C Shewanella sp.
2562
-
 pyruvate pH 10.0, 40°C Shewanella sp.
2871
-
 NADH pH 10.0, 40°C Shewanella sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Shewanella sp.

pI Value

Organism Comment pI Value Maximum pI Value
Shewanella sp. theoretical value
-
 4.9