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Literature summary for 1.4.1.1 extracted from
- Purification, properties and primary structure of alanine dehydrogenase involved in taurine metabolism in the anaerobe Bilophila wadsworthia (2000), Arch. Microbiol., 174, 162-167.
General Stability
| General Stability | Organism |
|---|---|
| repeated freezing and thawing leads to significant loss of activity | Bilophila wadsworthia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
NADH | - |
Bilophila wadsworthia |  |
| 0.15 | - |
NAD+ | - |
Bilophila wadsworthia | |
| 1.1 | - |
pyruvate | - |
Bilophila wadsworthia | |
| 1.6 | - |
L-Ala | - |
Bilophila wadsworthia | |
| 31 | - |
NH4+ | - |
Bilophila wadsworthia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39900 | - |
6 * 39900, calculation from nucleotide sequence | Bilophila wadsworthia |
| 42000 | - |
6 * 42000, SDS-PAGE | Bilophila wadsworthia |
| 273000 | - |
gel filtration | Bilophila wadsworthia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bilophila wadsworthia | enzyme is involved in taurine metabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bilophila wadsworthia | Q9AIK2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bilophila wadsworthia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bilophila wadsworthia |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 1 month | Bilophila wadsworthia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Ala + H2O + NAD+ | 3.5% of the activity with L-Ala | Bilophila wadsworthia | pyruvate + NH3 + NADH | - |
? | |
| D-Ser + H2O + NAD+ | 4.4% of the activity with L-Ala | Bilophila wadsworthia | 3-hydroxypyruvate + NH3 + NADH | - |
r | |
| D-Ser + H2O + NAD+ | in the reverse reaction the enzyme reacts at 9.3% of the activity with pyruvate | Bilophila wadsworthia | 3-hydroxypyruvate + NH3 + NADH | - |
r | |
| glyoxylate + NH3 + NADH | - |
Bilophila wadsworthia | aminoacetate + NAD+ + H2O | - |
? | |
| L-2-aminobutanoate + H2O + NAD+ | in the reverse reaction the enzyme reacts at 15.3% of the activity with pyruvate | Bilophila wadsworthia | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
| L-2-aminobutanoate + H2O + NAD+ | 10.4% of the activity with L-Ala | Bilophila wadsworthia | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
| L-Ala + H2O + NAD+ | - |
Bilophila wadsworthia | pyruvate + NH3 + NADH | - |
r | |
| L-Ile + H2O + NAD+ | - |
Bilophila wadsworthia | 2-oxo-3-methylpentanoate + NH3 + NADH | - |
? | |
| L-norvaline + H2O + NAD+ | at 4.6% of the activity with L-Ala | Bilophila wadsworthia | 2-oxopentanoate + NH3 + NADH | - |
? | |
| L-Val + H2O + NAD+ | 4.8% of the activity with L-Ala | Bilophila wadsworthia | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
| additional information | no activity with taurine | Bilophila wadsworthia | ? | - |
? | |
| additional information | enzyme is involved in taurine metabolism | Bilophila wadsworthia | ? | - |
? | |
| oxaloacetate + NH3 + NADH | - |
Bilophila wadsworthia | L-Asp + H2O + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 42000, SDS-PAGE | Bilophila wadsworthia |
| hexamer | 6 * 39900, calculation from nucleotide sequence | Bilophila wadsworthia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | oxidative deamination | Bilophila wadsworthia |
| 55 | 60 | reductive amination | Bilophila wadsworthia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
inactivation after about 1 min | Bilophila wadsworthia |
| 65 | - |
inactivation after about 10 s | Bilophila wadsworthia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
reductive amination of pyruvate | Bilophila wadsworthia |
| 9 | 11.5 | oxidative deamination of L-Ala | Bilophila wadsworthia |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Bilophila wadsworthia | |
| NADH | - |
Bilophila wadsworthia | |
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