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Literature summary for 1.4.1.1 extracted from
- Purification and properties of alanine dehydrogenase from Halobacterium salinarium (1979), Biochim. Biophys. Acta, 570, 1-10.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| F- | - |
Halobacterium salinarum |  |
| I- | - |
Halobacterium salinarum | |
| perchlorate | - |
Halobacterium salinarum | |
| thiocyanate | - |
Halobacterium salinarum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CdCl2 | stimulates | Halobacterium salinarum | |
| Cs+ | CsCl, weak stimulation | Halobacterium salinarum | |
| KCl | KCl stimulates | Halobacterium salinarum | |
| Li+ | LiCl, weak stimulation | Halobacterium salinarum | |
| additional information | highest activation by Cl-, much smaller effect occurs in presence of Br-, nitrate, or sulfate | Halobacterium salinarum | |
| NaCl | NaCl, active only in presence of high concentrations | Halobacterium salinarum | |
| RbCl | stimulates | Halobacterium salinarum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
non-denaturing PAGE | Halobacterium salinarum |
| 60000 | - |
gel filtration | Halobacterium salinarum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halobacterium salinarum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Halobacterium salinarum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 178 | - |
- |
Halobacterium salinarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxobutanoate + NH3 + NADH | - |
Halobacterium salinarum | 2-aminobutanoate + H2O + NAD+ | - |
r | |
| glyoxylate + NH3 + NADH | - |
Halobacterium salinarum | aminoacetate + NAD+ + H2O | - |
? | |
| hydroxypyruvate + NH3 + NADH | - |
Halobacterium salinarum | L-Ser + NAD+ + H2O | - |
? | |
| L-2-aminobutanoate + H2O + NAD+ | - |
Halobacterium salinarum | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
| L-Ala + H2O + NAD+ | - |
Halobacterium salinarum | pyruvate + NH3 + NADH | - |
r | |
| oxaloacetate + NH3 + NADH | - |
Halobacterium salinarum | L-Asp + H2O + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 58000-60000, SDS-PAGE | Halobacterium salinarum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
presence of 3.5 M NaNO3 | Halobacterium salinarum |
| 65 | - |
in presence of 3.5 M NaCl | Halobacterium salinarum |
| 70 | - |
3.5 M KCl | Halobacterium salinarum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal stability is considerably lower in presence of KCl than in presence of NaCl | Halobacterium salinarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
both directions | Halobacterium salinarum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Halobacterium salinarum | |
| NADH | - |
Halobacterium salinarum | |
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