Literature summary for 1.4.1.1 extracted from

Vali, Z.; Kilar, F.; Lakatos, S.; Venyaminov, S.A.; Zavodszky, P.
L-Alanine dehydrogenase from Thermus thermophilus (1980), Biochim. Biophys. Acta, 615, 34-47.

Search for more literature for 1.4.1.1

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Thermus thermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
D-Ala	-	Bacillus subtilis
D-Ala	-	Thermus thermophilus
D-Cys	-	Bacillus subtilis
D-Cys	-	Thermus thermophilus
L-Cys	-	Thermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.023	-	NADH	-	Bacillus subtilis
0.035	-	NADH	-	Bacillus subtilis
0.035	-	NADH	-	Thermus thermophilus
0.12	-	NAD+	-	Thermus thermophilus
0.18	-	NAD+	-	Bacillus subtilis
0.54	-	pyruvate	-	Bacillus subtilis
0.75	-	pyruvate	-	Thermus thermophilus
1.7	-	L-Ala	-	Bacillus subtilis
4.2	-	L-Ala	-	Thermus thermophilus
38	-	NH4+	-	Bacillus subtilis
59	-	NH3	-	Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	6 * 48000, SDS-PAGE	Thermus thermophilus
290000	-	equilibrium sedimentation	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	-	-
Thermus thermophilus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Ala + H2O + NAD+	-	Bacillus subtilis	pyruvate + NH3 + NADH	-	r
L-Ala + H2O + NAD+	-	Thermus thermophilus	pyruvate + NH3 + NADH	-	r
L-Ile + H2O + NAD+	0.1% of the activity with L-Ala	Thermus thermophilus	2-oxo-3-methylpentanoate + NH3 + NADH	-	?
L-Ile + H2O + NAD+	5% of the activity with L-Ala	Bacillus subtilis	2-oxo-3-methylpentanoate + NH3 + NADH	-	?
L-Ser + H2O + NAD+	-	Bacillus subtilis	3-hydroxypyruvate + NH3 + NADH	-	?
L-Ser + H2O + NAD+	-	Thermus thermophilus	3-hydroxypyruvate + NH3 + NADH	-	?
L-Val + H2O + NAD+	9% of the activity with L-Ala	Bacillus subtilis	3-methyl-2-oxobutanoate + NH3 + NADH	-	?
L-Val + H2O + NAD+	0.2% of the activity with L-Ala	Thermus thermophilus	3-methyl-2-oxobutanoate + NH3 + NADH	-	?

Subunits

Subunits	Comment	Organism
hexamer	-	Bacillus subtilis
hexamer	6 * 48000, SDS-PAGE	Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
63	-	melting temperature	Bacillus subtilis
86	-	melting temperature	Thermus thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Thermus thermophilus
8.5	-	-	Thermus thermophilus
8.5	-	reductive amination of pyruvate	Bacillus subtilis
10.5	-	deamination of L-Ala	Thermus thermophilus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Bacillus subtilis
NAD+	-	Thermus thermophilus
NADH	-	Bacillus subtilis
NADH	-	Thermus thermophilus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.64	-	D-Cys	-	Bacillus subtilis
0.9	-	D-Cys	-	Bacillus subtilis
5	-	D-Ala	-	Bacillus subtilis
20	-	D-Ala	-	Bacillus subtilis

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Release 2023.1 (January 2023)