Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | activation | Streptomyces phaeochromogenes | |
2,2'-dipyridyl | activation | Streptomyces phaeochromogenes | |
2-mercaptoethanol | activation | Streptomyces phaeochromogenes | |
diethyldithiocarbamate | activation | Streptomyces phaeochromogenes | |
dithiothreitol | activation | Streptomyces phaeochromogenes | |
EDTA | activation | Streptomyces phaeochromogenes |
Crystallization (Comment) | Organism |
---|---|
- |
Streptomyces phaeochromogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Lysinibacillus sphaericus | |
Cu2+ | - |
Streptomyces clavuligerus | |
Cu2+ | - |
Streptomyces phaeochromogenes | |
monoiodoacetate | - |
Streptomyces phaeochromogenes | |
Pb2+ | - |
Lysinibacillus sphaericus | |
Pb2+ | - |
Streptomyces phaeochromogenes | |
PCMB | - |
Bacillus subtilis | |
PCMB | - |
Lysinibacillus sphaericus | |
PCMB | - |
Streptomyces phaeochromogenes | |
Zn2+ | - |
Bacillus subtilis | |
Zn2+ | - |
Streptomyces clavuligerus | |
Zn2+ | - |
Streptomyces phaeochromogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
NAD+ | pH 10.0 | Streptomyces phaeochromogenes | |
0.047 | - |
NADH | - |
Streptomyces phaeochromogenes | |
0.29 | - |
pyruvate | - |
Streptomyces phaeochromogenes | |
1.9 | - |
L-Ala | pH 8.0 | Streptomyces phaeochromogenes | |
3.6 | - |
L-Ala | pH 9.0 | Streptomyces phaeochromogenes | |
7.1 | - |
L-Ala | pH 10.0 | Streptomyces phaeochromogenes | |
61 | - |
NH4+ | - |
Streptomyces phaeochromogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
6 * 39000, SDS-PAGE | Streptomyces phaeochromogenes |
240000 | - |
gel filtration | Streptomyces phaeochromogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Kitasatospora albolonga | - |
- |
- |
Lysinibacillus sphaericus | - |
- |
- |
Micromonospora melanosporea | - |
- |
- |
Streptomyces albus | - |
- |
- |
Streptomyces aureus | - |
- |
- |
Streptomyces bobili | - |
- |
- |
Streptomyces caespitosus | - |
- |
- |
Streptomyces clavuligerus | - |
- |
- |
Streptomyces coelicolor | - |
- |
- |
Streptomyces flaveus | - |
- |
- |
Streptomyces griseoluteus | - |
- |
- |
Streptomyces griseus | - |
- |
- |
Streptomyces hygroscopicus | - |
- |
- |
Streptomyces lydicus | - |
- |
- |
Streptomyces olivochromogenes | - |
- |
- |
Streptomyces phaeochromogenes | - |
- |
- |
Streptomyces roseochromogenus | - |
- |
- |
Streptomyces roseus | - |
- |
- |
Streptomyces ruber | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces phaeochromogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22.8 | - |
- |
Streptomyces phaeochromogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADH | - |
Lysinibacillus sphaericus | 2-aminobutanoate + H2O + NAD+ | - |
? | |
2-oxobutanoate + NH3 + NADH | 15.8% of the activity with pyruvate | Streptomyces phaeochromogenes | 2-aminobutanoate + H2O + NAD+ | - |
? | |
3-hydroxypyruvate + NH3 + NADH | - |
Lysinibacillus sphaericus | L-Ser + H2O + NAD+ | - |
? | |
3-hydroxypyruvate + NH3 + NADH | - |
Streptomyces clavuligerus | L-Ser + H2O + NAD+ | - |
? | |
3-hydroxypyruvate + NH3 + NADH | 32.3% of the activity with pyruvate | Streptomyces phaeochromogenes | L-Ser + H2O + NAD+ | - |
? | |
glyoxylate + NH3 + NADH | - |
Lysinibacillus sphaericus | aminoacetate + NAD+ + H2O | - |
? | |
glyoxylate + NH3 + NADH | - |
Streptomyces clavuligerus | aminoacetate + NAD+ + H2O | - |
? | |
glyoxylate + NH3 + NADH | 13.3% of the activity with pyruvate | Streptomyces phaeochromogenes | aminoacetate + NAD+ + H2O | - |
? | |
L-2-aminobutanoate + H2O + NAD+ | - |
Bacillus subtilis | 2-oxobutanoate + NH3 + NADH + H+ | - |
? | |
L-2-aminobutanoate + H2O + NAD+ | - |
Lysinibacillus sphaericus | 2-oxobutanoate + NH3 + NADH + H+ | - |
? | |
L-2-aminobutanoate + H2O + NAD+ | 1.6% of the activity with L-Ala | Streptomyces phaeochromogenes | 2-oxobutanoate + NH3 + NADH + H+ | - |
? | |
L-Ala + H2O + NAD+ | - |
Bacillus subtilis | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Lysinibacillus sphaericus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces coelicolor | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces griseus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces albus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces olivochromogenes | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces phaeochromogenes | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces flaveus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces bobili | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces roseochromogenus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces ruber | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces aureus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces lydicus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces hygroscopicus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces caespitosus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces clavuligerus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces griseoluteus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Kitasatospora albolonga | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Micromonospora melanosporea | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Streptomyces roseus | pyruvate + NH3 + NADH | - |
r | |
L-Ser + H2O + NAD+ | - |
Bacillus subtilis | 3-hydroxypyruvate + NH3 + NADH | - |
? | |
L-Ser + H2O + NAD+ | - |
Lysinibacillus sphaericus | 3-hydroxypyruvate + NH3 + NADH | - |
? | |
L-Ser + H2O + NAD+ | 2.4% of the activity with L-Ala | Streptomyces phaeochromogenes | 3-hydroxypyruvate + NH3 + NADH | - |
? | |
oxaloacetate + NH3 + NADH | - |
Lysinibacillus sphaericus | L-Asp + H2O + NAD+ | - |
? | |
oxaloacetate + NH3 + NADH | 43.1% of the activity with pyruvate | Streptomyces phaeochromogenes | L-Asp + H2O + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Bacillus subtilis |
hexamer | - |
Lysinibacillus sphaericus |
hexamer | 6 * 39000, SDS-PAGE | Streptomyces phaeochromogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Streptomyces phaeochromogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
20 min, pH 7.0, stable | Streptomyces phaeochromogenes |
50 | - |
- |
Lysinibacillus sphaericus |
50 | - |
- |
Streptomyces coelicolor |
50 | - |
20 min, pH 7.0, 29% loss of activity | Streptomyces phaeochromogenes |
55 | - |
20 min, pH 7.0, 50% loss of activity | Streptomyces phaeochromogenes |
60 | - |
- |
Lysinibacillus sphaericus |
60 | - |
20 min, pH 7.0, 70% loss of activity | Streptomyces phaeochromogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
reductive amination | Streptomyces phaeochromogenes |
10 | - |
oxidative deamination | Streptomyces phaeochromogenes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | 20°C, 3 h, stable | Streptomyces phaeochromogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis | |
NAD+ | - |
Lysinibacillus sphaericus | |
NAD+ | - |
Streptomyces coelicolor | |
NAD+ | - |
Streptomyces griseus | |
NAD+ | - |
Streptomyces albus | |
NAD+ | - |
Streptomyces olivochromogenes | |
NAD+ | - |
Streptomyces phaeochromogenes | |
NAD+ | - |
Streptomyces flaveus | |
NAD+ | - |
Streptomyces bobili | |
NAD+ | - |
Streptomyces roseochromogenus | |
NAD+ | - |
Streptomyces ruber | |
NAD+ | - |
Streptomyces aureus | |
NAD+ | - |
Streptomyces lydicus | |
NAD+ | - |
Streptomyces hygroscopicus | |
NAD+ | - |
Streptomyces caespitosus | |
NAD+ | - |
Streptomyces clavuligerus | |
NAD+ | - |
Streptomyces griseoluteus | |
NAD+ | - |
Kitasatospora albolonga | |
NAD+ | - |
Micromonospora melanosporea | |
NAD+ | - |
Streptomyces roseus | |
NADH | - |
Bacillus subtilis | |
NADH | - |
Lysinibacillus sphaericus | |
NADH | - |
Streptomyces coelicolor | |
NADH | - |
Streptomyces griseus | |
NADH | - |
Streptomyces albus | |
NADH | - |
Streptomyces olivochromogenes | |
NADH | - |
Streptomyces phaeochromogenes | |
NADH | - |
Streptomyces flaveus | |
NADH | - |
Streptomyces bobili | |
NADH | - |
Streptomyces roseochromogenus | |
NADH | - |
Streptomyces ruber | |
NADH | - |
Streptomyces aureus | |
NADH | - |
Streptomyces lydicus | |
NADH | - |
Streptomyces hygroscopicus | |
NADH | - |
Streptomyces caespitosus | |
NADH | - |
Streptomyces clavuligerus | |
NADH | - |
Streptomyces griseoluteus | |
NADH | - |
Kitasatospora albolonga | |
NADH | - |
Micromonospora melanosporea | |
NADH | - |
Streptomyces roseus | |
NADP+ | 0.6% of the activity with NAD+ | Streptomyces phaeochromogenes | |
NADPH | 3.5% of the activity with NADH | Streptomyces phaeochromogenes |