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Literature summary for 1.4.1.1 extracted from

  • Itoh, N.; Morikawa, R.; Itoh, N.; Morikawa, R.
    Crystallization and properties of L-alanine dehydrogenase from Streptomyces phaeochromogenes (1983), Agric. Biol. Chem., 47, 2511-2519.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
1,10-phenanthroline activation Streptomyces phaeochromogenes
2,2'-dipyridyl activation Streptomyces phaeochromogenes
2-mercaptoethanol activation Streptomyces phaeochromogenes
diethyldithiocarbamate activation Streptomyces phaeochromogenes
dithiothreitol activation Streptomyces phaeochromogenes
EDTA activation Streptomyces phaeochromogenes

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Streptomyces phaeochromogenes

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Lysinibacillus sphaericus
Cu2+
-
Streptomyces clavuligerus
Cu2+
-
Streptomyces phaeochromogenes
monoiodoacetate
-
Streptomyces phaeochromogenes
Pb2+
-
Lysinibacillus sphaericus
Pb2+
-
Streptomyces phaeochromogenes
PCMB
-
Bacillus subtilis
PCMB
-
Lysinibacillus sphaericus
PCMB
-
Streptomyces phaeochromogenes
Zn2+
-
Bacillus subtilis
Zn2+
-
Streptomyces clavuligerus
Zn2+
-
Streptomyces phaeochromogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
NAD+ pH 10.0 Streptomyces phaeochromogenes
0.047
-
NADH
-
Streptomyces phaeochromogenes
0.29
-
pyruvate
-
Streptomyces phaeochromogenes
1.9
-
L-Ala pH 8.0 Streptomyces phaeochromogenes
3.6
-
L-Ala pH 9.0 Streptomyces phaeochromogenes
7.1
-
L-Ala pH 10.0 Streptomyces phaeochromogenes
61
-
NH4+
-
Streptomyces phaeochromogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
6 * 39000, SDS-PAGE Streptomyces phaeochromogenes
240000
-
gel filtration Streptomyces phaeochromogenes

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Kitasatospora albolonga
-
-
-
Lysinibacillus sphaericus
-
-
-
Micromonospora melanosporea
-
-
-
Streptomyces albus
-
-
-
Streptomyces aureus
-
-
-
Streptomyces bobili
-
-
-
Streptomyces caespitosus
-
-
-
Streptomyces clavuligerus
-
-
-
Streptomyces coelicolor
-
-
-
Streptomyces flaveus
-
-
-
Streptomyces griseoluteus
-
-
-
Streptomyces griseus
-
-
-
Streptomyces hygroscopicus
-
-
-
Streptomyces lydicus
-
-
-
Streptomyces olivochromogenes
-
-
-
Streptomyces phaeochromogenes
-
-
-
Streptomyces roseochromogenus
-
-
-
Streptomyces roseus
-
-
-
Streptomyces ruber
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces phaeochromogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22.8
-
-
Streptomyces phaeochromogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NH3 + NADH
-
Lysinibacillus sphaericus 2-aminobutanoate + H2O + NAD+
-
?
2-oxobutanoate + NH3 + NADH 15.8% of the activity with pyruvate Streptomyces phaeochromogenes 2-aminobutanoate + H2O + NAD+
-
?
3-hydroxypyruvate + NH3 + NADH
-
Lysinibacillus sphaericus L-Ser + H2O + NAD+
-
?
3-hydroxypyruvate + NH3 + NADH
-
Streptomyces clavuligerus L-Ser + H2O + NAD+
-
?
3-hydroxypyruvate + NH3 + NADH 32.3% of the activity with pyruvate Streptomyces phaeochromogenes L-Ser + H2O + NAD+
-
?
glyoxylate + NH3 + NADH
-
Lysinibacillus sphaericus aminoacetate + NAD+ + H2O
-
?
glyoxylate + NH3 + NADH
-
Streptomyces clavuligerus aminoacetate + NAD+ + H2O
-
?
glyoxylate + NH3 + NADH 13.3% of the activity with pyruvate Streptomyces phaeochromogenes aminoacetate + NAD+ + H2O
-
?
L-2-aminobutanoate + H2O + NAD+
-
Bacillus subtilis 2-oxobutanoate + NH3 + NADH + H+
-
?
L-2-aminobutanoate + H2O + NAD+
-
Lysinibacillus sphaericus 2-oxobutanoate + NH3 + NADH + H+
-
?
L-2-aminobutanoate + H2O + NAD+ 1.6% of the activity with L-Ala Streptomyces phaeochromogenes 2-oxobutanoate + NH3 + NADH + H+
-
?
L-Ala + H2O + NAD+
-
Bacillus subtilis pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Lysinibacillus sphaericus pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces coelicolor pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces griseus pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces albus pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces olivochromogenes pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces phaeochromogenes pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces flaveus pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces bobili pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces roseochromogenus pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces ruber pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces aureus pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces lydicus pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces hygroscopicus pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces caespitosus pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces clavuligerus pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces griseoluteus pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Kitasatospora albolonga pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Micromonospora melanosporea pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Streptomyces roseus pyruvate + NH3 + NADH
-
r
L-Ser + H2O + NAD+
-
Bacillus subtilis 3-hydroxypyruvate + NH3 + NADH
-
?
L-Ser + H2O + NAD+
-
Lysinibacillus sphaericus 3-hydroxypyruvate + NH3 + NADH
-
?
L-Ser + H2O + NAD+ 2.4% of the activity with L-Ala Streptomyces phaeochromogenes 3-hydroxypyruvate + NH3 + NADH
-
?
oxaloacetate + NH3 + NADH
-
Lysinibacillus sphaericus L-Asp + H2O + NAD+
-
?
oxaloacetate + NH3 + NADH 43.1% of the activity with pyruvate Streptomyces phaeochromogenes L-Asp + H2O + NAD+
-
?

Subunits

Subunits Comment Organism
hexamer
-
Bacillus subtilis
hexamer
-
Lysinibacillus sphaericus
hexamer 6 * 39000, SDS-PAGE Streptomyces phaeochromogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Streptomyces phaeochromogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
20 min, pH 7.0, stable Streptomyces phaeochromogenes
50
-
-
Lysinibacillus sphaericus
50
-
-
Streptomyces coelicolor
50
-
20 min, pH 7.0, 29% loss of activity Streptomyces phaeochromogenes
55
-
20 min, pH 7.0, 50% loss of activity Streptomyces phaeochromogenes
60
-
-
Lysinibacillus sphaericus
60
-
20 min, pH 7.0, 70% loss of activity Streptomyces phaeochromogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
reductive amination Streptomyces phaeochromogenes
10
-
oxidative deamination Streptomyces phaeochromogenes

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10 20°C, 3 h, stable Streptomyces phaeochromogenes

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus subtilis
NAD+
-
Lysinibacillus sphaericus
NAD+
-
Streptomyces coelicolor
NAD+
-
Streptomyces griseus
NAD+
-
Streptomyces albus
NAD+
-
Streptomyces olivochromogenes
NAD+
-
Streptomyces phaeochromogenes
NAD+
-
Streptomyces flaveus
NAD+
-
Streptomyces bobili
NAD+
-
Streptomyces roseochromogenus
NAD+
-
Streptomyces ruber
NAD+
-
Streptomyces aureus
NAD+
-
Streptomyces lydicus
NAD+
-
Streptomyces hygroscopicus
NAD+
-
Streptomyces caespitosus
NAD+
-
Streptomyces clavuligerus
NAD+
-
Streptomyces griseoluteus
NAD+
-
Kitasatospora albolonga
NAD+
-
Micromonospora melanosporea
NAD+
-
Streptomyces roseus
NADH
-
Bacillus subtilis
NADH
-
Lysinibacillus sphaericus
NADH
-
Streptomyces coelicolor
NADH
-
Streptomyces griseus
NADH
-
Streptomyces albus
NADH
-
Streptomyces olivochromogenes
NADH
-
Streptomyces phaeochromogenes
NADH
-
Streptomyces flaveus
NADH
-
Streptomyces bobili
NADH
-
Streptomyces roseochromogenus
NADH
-
Streptomyces ruber
NADH
-
Streptomyces aureus
NADH
-
Streptomyces lydicus
NADH
-
Streptomyces hygroscopicus
NADH
-
Streptomyces caespitosus
NADH
-
Streptomyces clavuligerus
NADH
-
Streptomyces griseoluteus
NADH
-
Kitasatospora albolonga
NADH
-
Micromonospora melanosporea
NADH
-
Streptomyces roseus
NADP+ 0.6% of the activity with NAD+ Streptomyces phaeochromogenes
NADPH 3.5% of the activity with NADH Streptomyces phaeochromogenes