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Literature summary for 1.4.1.1 extracted from

  • Bellion, E.; Tan, F.
    An NAD+-dependent alanine dehydrogenase from a methylotrophic bacterium (1987), Biochem. J., 244, 565-570.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.1

Inhibitors

Inhibitors Comment Organism Structure
3-Bromopyruvate
-
 Pseudomonas sp.
5,5'-dithiobis(2-nitrobenzoate)
-
 Pseudomonas sp.
HgCl2
-
 Pseudomonas sp.
Hydroxypyruvate
-
 Pseudomonas sp.
L-Ala
-
 Pseudomonas sp.
L-Ser
-
 Pseudomonas sp.
NAD+ 0.1 mM, 35% inhibition Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 4 * 53000, SDS-PAGE Pseudomonas sp.
217000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Ala + H2O + NAD+ Pseudomonas sp. role in NH3 assimilation and alanine catabolism pyruvate + NH3 + NADH
-
 ?
L-Ala + H2O + NAD+ Pseudomonas sp. MA role in NH3 assimilation and alanine catabolism pyruvate + NH3 + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. MA
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
129
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala + H2O + NAD+ rate of the deaminating reaction is only 2.2% of the aminating reaction Pseudomonas sp. pyruvate + NH3 + NADH
-
 r
L-Ala + H2O + NAD+ role in NH3 assimilation and alanine catabolism Pseudomonas sp. pyruvate + NH3 + NADH
-
 ?
L-Ala + H2O + NAD+ rate of the deaminating reaction is only 2.2% of the aminating reaction Pseudomonas sp. MA pyruvate + NH3 + NADH
-
 r
L-Ala + H2O + NAD+ role in NH3 assimilation and alanine catabolism Pseudomonas sp. MA pyruvate + NH3 + NADH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 53000, SDS-PAGE Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 reductive amination Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas sp.
NADH
-
 Pseudomonas sp.