Cloned (Comment) | Organism |
---|---|
cloned and expressed in Escherichia coli | Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39465 | - |
6 * 39465, calculation from nucleotide sequence | Lysinibacillus sphaericus |
39500 | - |
6 * 39500, recombinant enzyme, SDS-PAGE | Lysinibacillus sphaericus |
235000 | - |
recombinant enzyme, gel filtration | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Lysinibacillus sphaericus | - |
gene cloned and expressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala + H2O + NAD+ | - |
Geobacillus stearothermophilus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Lysinibacillus sphaericus | pyruvate + NH3 + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Geobacillus stearothermophilus |
hexamer | 6 * 39500, recombinant enzyme, SDS-PAGE | Lysinibacillus sphaericus |
hexamer | 6 * 39465, calculation from nucleotide sequence | Lysinibacillus sphaericus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
5 min, 50% loss of activity | Lysinibacillus sphaericus |
85 | - |
5 min, 50% loss of activity | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Geobacillus stearothermophilus | |
NAD+ | - |
Lysinibacillus sphaericus | |
NADH | - |
Geobacillus stearothermophilus | |
NADH | - |
Lysinibacillus sphaericus |