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Literature summary for 1.3.99.8 extracted from

  • Kitcher, J.P.; Trudgill, P.W.; Rees, J.S.
    Purification and properties of 2-furoyl-coenzyme A hydroxylase from Pseudomonas putida F2 (1972), Biochem. J., 130, 121-132.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.99.8

Inhibitors

Inhibitors Comment Organism Structure
Bathocuproin
-
 Pseudomonas putida
Cuprizone
-
 Pseudomonas putida
cyanide not Pseudomonas putida
additional information not inhibitory: diethyldithiocarbamate, p-chloromercuribenzoate, arsenite, iodoacetamide, 2-n-heptyl-4-hydroxyquinoline N-oxide, azide, alpha,alpha'-bipyridyl, Tiron (i.e. disodium 1,2-dihydroxybenzene-3,5-disulfonate), 8-hydroxyquinoline, menadione Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02 0.05 2-Furoyl-CoA
-
 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
copper
-
 Pseudomonas putida
additional information no Fe Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3270000
-
 sedimentation equilibrium Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-furoyl-CoA + H2O + acceptor Pseudomonas putida
-
 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
2-furoyl-CoA + H2O + acceptor Pseudomonas putida F2
-
 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida F2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Reaction

Reaction Comment Organism Reaction ID
2-furoyl-CoA + H2O + acceptor = S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor mechanism Pseudomonas putida
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-furoyl-CoA + H2O + acceptor
-
 Pseudomonas putida S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
2-furoyl-CoA + H2O + acceptor acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida Pseudomonas putida S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor product is tautomerized to 5-oxo-DELTA2-dihydro-2-furoyl-CoA ?
2-furoyl-CoA + H2O + acceptor
-
 Pseudomonas putida F2 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
2-furoyl-CoA + H2O + acceptor acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida Pseudomonas putida F2 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor product is tautomerized to 5-oxo-DELTA2-dihydro-2-furoyl-CoA ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9.5 2-furoyl-CoA + methylene blue Pseudomonas putida