BRENDA - Enzyme Database show
show all sequences of 1.3.99.6

Purification and mechanism of action of a steroid delta-4-5-beta-dehydrogenase

Davidson, S.J.; Talalay, P.; J. Biol. Chem. 241, 906-915 (1966)

Data extracted from this reference:

General Stability
General Stability
Organism
glycerol stabilizes
Comamonas testosteroni
phenazine methosulfate stabilizes
Comamonas testosteroni
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
gel filtration
Comamonas testosteroni
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Comamonas testosteroni
-
-
-
Purification (Commentary)
Commentary
Organism
-
Comamonas testosteroni
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.5
-
-
Comamonas testosteroni
Storage Stability
Storage Stability
Organism
4°C, in neutral buffer, 20% glycerol, about 50% loss of activity after 1 month
Comamonas testosteroni
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17beta-hydroxy-5beta-androstan-3-one + menaphthone
acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10
391402
Comamonas testosteroni
testosterone + reduced acceptor
-
-
-
?
21-hydroxy-5beta-pregnan-3,20-dione + acceptor
acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10
391402
Comamonas testosteroni
21-hydroxy-pregn-4-en-3,20-dione + reduced acceptor
-
-
-
?
5beta-androst-3,17-dione + acceptor
acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10
391402
Comamonas testosteroni
androst-1,4-dien-3,17-dione + reduced acceptor
-
-
-
?
5beta-androstan-3,17-dione + acceptor
acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10
391402
Comamonas testosteroni
4-androsten-3,17-dione + reduced acceptor
-
-
-
?
5beta-androstane-3,17-dione + acceptor
acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10
391402
Comamonas testosteroni
androst-4-en-3,17-dione + reduced acceptor
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
above pH 9.0, the rate of DELTA4-5beta-dehydrogenase reaction increases with pH to the limit where accurate measurement can be made, pH 9.0
Comamonas testosteroni
General Stability (protein specific)
General Stability
Organism
glycerol stabilizes
Comamonas testosteroni
phenazine methosulfate stabilizes
Comamonas testosteroni
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
gel filtration
Comamonas testosteroni
Purification (Commentary) (protein specific)
Commentary
Organism
-
Comamonas testosteroni
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.5
-
-
Comamonas testosteroni
Storage Stability (protein specific)
Storage Stability
Organism
4°C, in neutral buffer, 20% glycerol, about 50% loss of activity after 1 month
Comamonas testosteroni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17beta-hydroxy-5beta-androstan-3-one + menaphthone
acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10
391402
Comamonas testosteroni
testosterone + reduced acceptor
-
-
-
?
21-hydroxy-5beta-pregnan-3,20-dione + acceptor
acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10
391402
Comamonas testosteroni
21-hydroxy-pregn-4-en-3,20-dione + reduced acceptor
-
-
-
?
5beta-androst-3,17-dione + acceptor
acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10
391402
Comamonas testosteroni
androst-1,4-dien-3,17-dione + reduced acceptor
-
-
-
?
5beta-androstan-3,17-dione + acceptor
acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10
391402
Comamonas testosteroni
4-androsten-3,17-dione + reduced acceptor
-
-
-
?
5beta-androstane-3,17-dione + acceptor
acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10
391402
Comamonas testosteroni
androst-4-en-3,17-dione + reduced acceptor
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
above pH 9.0, the rate of DELTA4-5beta-dehydrogenase reaction increases with pH to the limit where accurate measurement can be made, pH 9.0
Comamonas testosteroni
Other publictions for EC 1.3.99.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
676039
Sheenan
5beta-Dihydroprogesterone and ...
Homo sapiens
Mol. Hum. Reprod.
11
495-501
2005
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2
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5
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5
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1
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655692
Lemonde
Mutations in SRD5B1 (AKR1D1), ...
Homo sapiens
Gut
52
1494-1499
2003
-
-
1
-
3
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1
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2
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1
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1
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1
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3
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1
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1
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391405
Ando
Expression of cholesterol 7alp ...
Rattus norvegicus
J. Lipid Res.
40
1793-1798
1999
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389552
Gao
Purification and properties of ...
Pseudomonas sp.
Ann. N. Y. Acad. Sci.
864
383-392
1998
-
1
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1
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1
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2
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1
1
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1
1
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391404
Zhu
Identification of the 37-kd ra ...
Rattus norvegicus
Hepatology
23
115-122
1996
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1
-
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
287079
Stokes
Characterization of DELTA4-3-k ...
Clostridium innocuum, Clostridium paraputrificum
Biochim. Biophys. Acta
836
255-261
1985
2
-
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1
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1
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2
2
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1
3
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2
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2
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1
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1
3
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1
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-
391381
Aries
-
Degradation of steroids by int ...
Clostridium paraputrificum
Biochim. Biophys. Acta
248
482-488
1971
-
-
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5
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1
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1
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12
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1
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1
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5
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1
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12
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1
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1
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391402
Davidson
Purification and mechanism of ...
Comamonas testosteroni
J. Biol. Chem.
241
906-915
1966
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2
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1
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1
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1
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1
1
5
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1
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2
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1
5
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1
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