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Literature summary for 1.3.99.4 extracted from

  • Shao, M.; Zhang, X.; Rao, Z.; Xu, M.; Yang, T.; Li, H.; Xu, Z.; Yang, S.
    A mutant form of 3-ketosteroid-Delta(1)-dehydrogenase gives altered androst-1,4-diene-3, 17-dione/androst-4-ene-3,17-dione molar ratios in steroid biotransformations by Mycobacterium neoaurum ST-095 (2016), J. Ind. Microbiol. Biotechnol., 43, 691-701 .
    View publication on PubMed
Search for more literature for 1.3.99.4

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mycolicibacterium neoaurum

Protein Variants

Protein Variants Comment Organism
additional information enzyme from strain ST-095 only differs in residue V366 from strain JC-12 (S366). Mutation results in almost 3fold higher catalytic efficiency in strain JC-12 Mycolicibacterium neoaurum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 4-androstene-3,17-dione pH 7.0, 30°C Mycolicibacterium neoaurum
0.034
-
 4-androstene-3,17-dione pH 7.0, 30°C Mycolicibacterium neoaurum

Organism

Organism UniProt Comment Textmining
Mycolicibacterium neoaurum
-
-
-
Mycolicibacterium neoaurum JC-12
-
-
-
Mycolicibacterium neoaurum ST-095
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.46
-
 pH 7.0, 30°C Mycolicibacterium neoaurum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Mycolicibacterium neoaurum 1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Mycolicibacterium neoaurum JC-12 1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Mycolicibacterium neoaurum ST-095 1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Mycolicibacterium neoaurum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 4-androstene-3,17-dione pH 7.0, 30°C Mycolicibacterium neoaurum
0.096
-
 4-androstene-3,17-dione pH 7.0, 30°C Mycolicibacterium neoaurum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.17
-
 4-androstene-3,17-dione pH 7.0, 30°C Mycolicibacterium neoaurum
8.83
-
 4-androstene-3,17-dione pH 7.0, 30°C Mycolicibacterium neoaurum