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Literature summary for 1.3.99.4 extracted from
- Characterization and application of fusidane antibiotic biosynethsis enzyme 3-ketosteroid-DELTA1-dehydrogenase in steroid transformation (2012), Appl. Microbiol. Biotechnol., 96, 133-142.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | kstDF is a promising enzyme in steroid DELTA1-dehydrogenation and steroid reduction that is propitious to construct genetically engineered steroid-transforming recombinants by heterologous overexpression | Aspergillus fumigatus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene kstDF, DNA and amino acid sequence determination, analysis, and comparison, expression in Escherichia coli strain BL21(lambdaDE3) and in Pichia pastoris strains KM71 and GS115 cytoplasm, both intracellularly and extracelluarly, the latter is inactive, method optimization | Aspergillus fumigatus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Aspergillus fumigatus | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64370 | - |
x * 64370, sequence calculation | Aspergillus fumigatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-androstene-3,17-dione + FAD | Aspergillus fumigatus | - |
1,4-androstadiene-3,17-dione + FADH2 | - |
r |  |
| 4-androstene-3,17-dione + FAD | Aspergillus fumigatus CICC 40167 | - |
1,4-androstadiene-3,17-dione + FADH2 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | - |
gene kstDF | - |
| Aspergillus fumigatus CICC 40167 | - |
gene kstDF | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Pichia pastoris GS115 by hydrophobic interaction chromatography, recombinant His-tagged enzyme from Escherichia coli strain BL21(lambdaDE3) by nickel affinity chromatography | Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-androstene-3,17-dione + FAD | - |
Aspergillus fumigatus | 1,4-androstadiene-3,17-dione + FADH2 | - |
r | |
| 4-androstene-3,17-dione + FAD | - |
Aspergillus fumigatus CICC 40167 | 1,4-androstadiene-3,17-dione + FADH2 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64370, sequence calculation | Aspergillus fumigatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-ketosteroid-DELTA1-dehydrogenase | - |
Aspergillus fumigatus |
| 4-ene-3-oxosteroid:(acceptor)l-ene-oxidoreductase | - |
Aspergillus fumigatus |
| KstDF | - |
Aspergillus fumigatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Aspergillus fumigatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Aspergillus fumigatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Aspergillus fumigatus | |
| FADH2 | - |
Aspergillus fumigatus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus fumigatus | sequence calculation | - |
7.81 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme functions in the conversion of 4-ene-3-oxosteroid to 1,4-diene-3-oxosteroid by trans-axial elimination of the Cl and C2 hydrogens, it is active in DELTA1-dehydrogenation in fusidane antibiotic biosynthesis, an important transformations in the synthesis of steroid hormones | Aspergillus fumigatus |
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