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Literature summary for 1.3.99.4 extracted from

  • Rohman, A.; van Oosterwijk, N.; Dijkstra, B.W.
    Purification, crystallization and preliminary X-ray crystallographic analysis of 3-ketosteroid DELTA1-dehydrogenase from Rhodococcus erythropolis SQ1 (2012), Acta Crystallogr. Sect. F, 68, 551-556.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene kstD1, heterologous expression of the His-tagged DELTA1-KSTD1 protein in Escherichia coli strain BL21(DE3) Rhodococcus erythropolis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method, mixing of 15 mg/ml protein in 25 mM bicine, pH 9.0, 100 mM NaCl, 10% v/v glycerol with 2% v/v PEG 400, 0.1 M, HEPES, pH 7.5, 2.0 M ammonium sulfate, 20°C, 5-7 days, method optimization, X-ray diffraction structure determination and analysis at 2.0 A resolution, multiwavelength anomalous dispersion analysis Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
x * 56000 Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-androstene-3,17-dione + FAD Rhodococcus erythropolis
-
1,4-androstadiene-3,17-dione + FADH2
-
?
4-androstene-3,17-dione + FAD Rhodococcus erythropolis SQ1
-
1,4-androstadiene-3,17-dione + FADH2
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis Q9RA02 three 3-ketosteroid DELTA1-dehydrogenase isoenzymes, gene kstD1 encodes isozyme DELTA1-KSTD1
-
Rhodococcus erythropolis SQ1 Q9RA02 three 3-ketosteroid DELTA1-dehydrogenase isoenzymes, gene kstD1 encodes isozyme DELTA1-KSTD1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged DELTA1-KSTD1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-androstene-3,17-dione + FAD
-
Rhodococcus erythropolis 1,4-androstadiene-3,17-dione + FADH2
-
?
4-androstene-3,17-dione + FAD
-
Rhodococcus erythropolis SQ1 1,4-androstadiene-3,17-dione + FADH2
-
?

Subunits

Subunits Comment Organism
? x * 56000 Rhodococcus erythropolis

Synonyms

Synonyms Comment Organism
3-ketosteroid delta1-dehydrogenase
-
Rhodococcus erythropolis
DELTA1-KSTD1
-
Rhodococcus erythropolis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 below pH 6.0 no apparent Tm is observed, indicating that DELTA1-KSTD1 is destabilized at low pH. The higher the pH value, within the range of pH 6.0-9.0, the higher the apparent Tm of DELTA1-KSTD1. At pH 9.0 the apparent Tm of DELTA1-KSTD1 is about 7 K higher than at pH 7.0 Rhodococcus erythropolis

Cofactor

Cofactor Comment Organism Structure
FAD prosthetic group, flavoenzyme Rhodococcus erythropolis

General Information

General Information Comment Organism
physiological function 3-ketosteroid DELTA1-dehydrogenase plays a crucial role in the early steps of steroid degradation by introducing a double bond between the C1 and C2 atoms of the A-ring of its 3-ketosteroid substrates Rhodococcus erythropolis