Literature summary for 1.3.99.4 extracted from

Li, Y.; Lu, F.; Sun, T.; Du, L.
Expression of ksdD gene encoding 3-ketosteroid-DELTA1-dehydrogenase from Arthrobacter simplex in Bacillus subtilis (2007), Lett. Appl. Microbiol., 44, 563-568.

Search for more literature for 1.3.99.4

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Bacillus subtilis strain WB600	Pimelobacter simplex

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	SDS-PAGE	Pimelobacter simplex

Organism

Organism	UniProt	Comment	Textmining
Pimelobacter simplex	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.000015	-	intracellular, soluble, recombinant enzyme, in 0.86 M Tris-HCl (pH9.0), 150 mM phenazine methosulfate, at 25°C, using 4-androstene-3,17-dione as substrate	Pimelobacter simplex
0.000111	-	exracellular, soluble, recombinant enzyme, in 0.86 M Tris-HCl (pH9.0), 150 mM phenazine methosulfate, at 25°C, using 4-androstene-3,17-dione as substrate	Pimelobacter simplex

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-androstene-3,17-dione + phenazine methosulfate	-	Pimelobacter simplex	1,4-androstene-3,17-dione + reduced phenazine methosulfate	-	?

Synonyms

Synonyms	Comment	Organism
3-ketosteroid-DELTA1-dehydrogenase	-	Pimelobacter simplex
KSDH	-	Pimelobacter simplex

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)