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Literature summary for 1.3.99.4 extracted from
- 3-Keto-5alpha-steroid DELTA(1)-dehydrogenase from Rhodococcus erythropolis SQ1 and its orthologue in Mycobacterium tuberculosis H37Rv are highly specific enzymes that function in cholesterol catabolism (2008), Biochem. J., 410, 339-346.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Rhodococcus erythropolis |
General Stability
| General Stability | Organism |
|---|---|
| KSTD3 activity is lost upon purification using Ni-NTA column chromatography | Rhodococcus erythropolis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
progesterone | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis |  |
| 0.005 | - |
4-androstene-3,17-dione | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.006 | - |
5alpha-androstan-3-one-17beta-ol | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.007 | - |
5alpha-pregnane-3,20-dione | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.013 | - |
5alpha-androstane-3,17-dione | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.014 | - |
5alpha-pregnane-3,20-dione | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.018 | - |
progesterone | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.021 | - |
5alpha-pregnane-3,20-dione | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.026 | - |
23,24-bis-nor-5alpha-cholestan-3-one acid | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.029 | - |
progesterone | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.033 | - |
5alpha-androstane-3,17-dione | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Mycobacterium tuberculosis | |
| 0.036 | - |
5alpha-androstane-3,17-dione | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.036 | - |
5alpha-androstan-3-one-17beta-ol | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.058 | - |
9alpha-hydroxy-4-androstene-3,17-dione | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.059 | - |
5alpha-androstane-3,17-dione | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.064 | - |
11beta-cortisol | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.065 | - |
progesterone | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Mycobacterium tuberculosis | |
| 0.07 | - |
5alpha-androstan-3-one-17beta-ol | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.074 | - |
23,24-bis-nor-5alpha-cholestan-3-one acid | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.104 | - |
4-androstene-3,17-dione | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.119 | - |
9alpha-hydroxy-4-androstene-3,17-dione | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
| 0.166 | - |
5alpha-androstan-3-one-17beta-ol | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Mycobacterium tuberculosis | |
| 0.557 | - |
11beta-cortisol | apparent value, in 50 mM Tris (pH 7.4), at 30°C | Rhodococcus erythropolis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
SDS-PAGE | Rhodococcus erythropolis |
| 60000 | - |
SDS-PAGE | Rhodococcus erythropolis |
| 61000 | - |
SDS-PAGE | Rhodococcus erythropolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
| Rhodococcus erythropolis | A9QAE5 | isozyme KSTD3 | - |
| Rhodococcus erythropolis | Q8RLT5 | isozyme KSTD2 | - |
| Rhodococcus erythropolis | Q9RA02 | isozyme KSTD1 | - |
| Rhodococcus erythropolis SQ1 | A9QAE5 | isozyme KSTD3 | - |
| Rhodococcus erythropolis SQ1 | Q8RLT5 | isozyme KSTD2 | - |
| Rhodococcus erythropolis SQ1 | Q9RA02 | isozyme KSTD1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| KSTD3 activity is lost upon purification using Ni-NTA column chromatography | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 11beta-cortisol + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis | (11beta)-11,17,21-trihydroxypregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 11beta-cortisol + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis SQ1 | (11beta)-11,17,21-trihydroxypregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 23,24-bis-nor-5alpha-cholestan-3-one acid + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis | ? | - |
? | |
| 23,24-bis-nor-5alpha-cholestan-3-one acid + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis SQ1 | ? | - |
? | |
| 4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis | 1,4-androstene-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis SQ1 | 1,4-androstene-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 4-pregnene-3,20-dione + acceptor | - |
Rhodococcus erythropolis | 1,4-pregnene-3,20 dione + reduced acceptor | - |
? | |
| 4-pregnene-3,20-dione + acceptor | - |
Rhodococcus erythropolis SQ1 | 1,4-pregnene-3,20 dione + reduced acceptor | - |
? | |
| 5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis | (5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol | KSTD3 shows highest activity on 5alpha-androstan-3-one-17beta-ol which is also known as 17beta-hydroxy-5alpha-androstane-3-one | Rhodococcus erythropolis | (5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol | KSTD3 shows highest activity on 5alpha-testosterone which is also known as 17beta-hydroxy-5alpha-androstane-3-one | Mycobacterium tuberculosis | (5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol | KSTD3 shows highest activity on 5alpha-androstan-3-one-17beta-ol which is also known as 17beta-hydroxy-5alpha-androstane-3-one | Rhodococcus erythropolis SQ1 | (5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis SQ1 | (5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol | KSTD3 shows highest activity on 5alpha-testosterone which is also known as 17beta-hydroxy-5alpha-androstane-3-one | Mycobacterium tuberculosis H37Rv | (5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis | 5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol | KSTD3 shows highest activity with 5alpha-androstane-3,17-dione | Mycobacterium tuberculosis | 5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol | KSTD3 shows highest activity with 5alpha-androstane-3,17-dione | Rhodococcus erythropolis | 5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol | KSTD3 shows highest activity with 5alpha-androstane-3,17-dione | Rhodococcus erythropolis SQ1 | 5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol | KSTD3 shows highest activity with 5alpha-androstane-3,17-dione | Mycobacterium tuberculosis H37Rv | 5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 5alpha-pregnane-3,20-dione + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis | 5alpha-pregn-1-en-3,20-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 5alpha-pregnane-3,20-dione + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis SQ1 | 5alpha-pregn-1-en-3,20-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis | 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol | preferred substrate of isozyme KSTD2 | Rhodococcus erythropolis | 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis | 9alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| additional information | 4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates | Rhodococcus erythropolis | ? | - |
? | |
| additional information | 4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bisnor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 5alpha-pregnane-3,20-dione, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol, 17-one are no substrates | Mycobacterium tuberculosis | ? | - |
? | |
| additional information | 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates | Rhodococcus erythropolis | ? | - |
? | |
| additional information | 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates | Rhodococcus erythropolis | ? | - |
? | |
| additional information | 4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates | Rhodococcus erythropolis SQ1 | ? | - |
? | |
| additional information | 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates | Rhodococcus erythropolis SQ1 | ? | - |
? | |
| additional information | 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates | Rhodococcus erythropolis SQ1 | ? | - |
? | |
| additional information | 4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bisnor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 5alpha-pregnane-3,20-dione, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol, 17-one are no substrates | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| progesterone + 2,6-dichlorophenol-indophenol | - |
Mycobacterium tuberculosis | pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| progesterone + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis | pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| progesterone + 2,6-dichlorophenol-indophenol | - |
Mycobacterium tuberculosis H37Rv | pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-keto-5alpha-steroid DELTA(1)-dehydrogenase | - |
Mycobacterium tuberculosis |
| 3-keto-5alpha-steroid DELTA(1)-dehydrogenase | - |
Rhodococcus erythropolis |
| 4-ene-3-oxosteroid: (acceptor)-1-ene-oxoreductase | - |
Mycobacterium tuberculosis |
| 4-ene-3-oxosteroid: (acceptor)-1-ene-oxoreductase | - |
Rhodococcus erythropolis |
| KSTD1 | isozyme | Rhodococcus erythropolis |
| KSTD2 | isozyme | Rhodococcus erythropolis |
| KSTD3 | isozyme | Rhodococcus erythropolis |
| KSTD3 orthologue | - |
Mycobacterium tuberculosis |
| Rv3537 | - |
Mycobacterium tuberculosis |
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