Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | - |
Pimelobacter simplex | |
CuCl2 | - |
Pimelobacter simplex | |
HgCl2 | - |
Pimelobacter simplex | |
additional information | not sensitive to carbonyl reagents and little sensitivity to metal chelating agents | Pimelobacter simplex | |
p-chloromercuribenzoate | - |
Pimelobacter simplex |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.071 | - |
4-androstene-3,17-dione | - |
Pimelobacter simplex |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE, gel filtration | Pimelobacter simplex |
Organic Solvent | Comment | Organism |
---|---|---|
Glycerol | 10%, stabilization | Pimelobacter simplex |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pimelobacter simplex | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pimelobacter simplex |
Storage Stability | Organism |
---|---|
4°C, 10% glycerol, at least 3 months stable | Pimelobacter simplex |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,11,17-trione + acceptor | - |
Pimelobacter simplex | 1,4-androstene-3,11,17-trione + reduced acceptor | - |
? | |
4-androstene-3,17-dione + acceptor | - |
Pimelobacter simplex | 1,4-androstene-3,17-dione + reduced acceptor | - |
? | |
4-pregnene-3,20-dione + acceptor | - |
Pimelobacter simplex | 1,4-pregnene-3,20 dione + reduced acceptor | - |
? | |
cortisone + acceptor | - |
Pimelobacter simplex | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor | - |
? | |
testosterone + acceptor | - |
Pimelobacter simplex | 17beta-hydroxyandrost-1,4-diene-3-one + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE, PAGE, gel filtration, N-terminal sequence | Pimelobacter simplex |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pimelobacter simplex |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 40 | maximum at 40°C, enzyme stable below 40°C | Pimelobacter simplex |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
substrate 4-androstene-3,17-dione, buffer 0.1 M glycine-NaOH | Pimelobacter simplex |