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Literature summary for 1.3.99.31 extracted from

  • Namitha, K.; Negi, P.
    Transformation of tomato cv. Arka Ahuti (Solanum lycopersicum L.) with phytoene desaturase (CrtI) and lycopene beta-cyclase (CrtY) genes increases carotenoid content and antioxidant potential (2018), J. Plant Biochem. Biotechnol., 27, 68-77 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene crtI, recombinant expression of the enzyme driven by 35S promoter in via Agrobacterium tumefaciens strain GV 3101 containing binary vector pRI 101(I) in fruits of Solanum tuberosum, coexpression of genes CrtI and CrtY, the latter encoding lycopene beta-cyclase, in transgenic tomato fruits, the method used is agroinjection of tomato fruits, a method of directly injecting Agrobacterium containing transgenes into fruits Pantoea agglomerans

Protein Variants

Protein Variants Comment Organism
additional information transformation of Solanum lycopersicum cv. Arka Ahuti with phytoene desaturase (CrtI) and lycopene beta-cyclase (CrtY) genes increases carotenoid content and antioxidant potential. coexpression of genes CrtI and CrtY, the latter encoding lycopene beta-cyclase, in transgenic tomato fruits, the method used is agroinjection of tomato fruits, a method of directly injecting Agrobacterium containing transgenes into fruits. The lycopene and beta-carotene content in green house grown tomatoes show a gradual increase from BR3 to BR7 stage, whereas in open-field grown tomatoes, the gradual increase is observed from BR3 to BR10 stage. Comparison of total antioxidant capacity of control and transformed tomatoes evaluated by phosphomolybdenum methodreveals higher total antioxidant capacity in the hydrophilic and lipophilic hexane as well as lipophilic acetone extracts in tomatoes with enhanced carotene content (CrtI and CrtY) fruits as compared to control tomato samples (GHC and OC), phenotypes, overview Pantoea agglomerans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
15-cis-phytoene + acceptor Pantoea agglomerans
-
all-trans-phytofluene + reduced acceptor
-
?
all-trans-neurosporene + acceptor Pantoea agglomerans
-
all-trans-lycopene + reduced acceptor
-
?
all-trans-phytofluene + acceptor Pantoea agglomerans
-
all-trans-zeta-carotene + reduced acceptor
-
?
all-trans-zeta-carotene + acceptor Pantoea agglomerans
-
all-trans-neurosporene + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Pantoea agglomerans Q206M4 Erwinia herbicola or Pantoea agglomerans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
15-cis-phytoene + acceptor
-
Pantoea agglomerans all-trans-phytofluene + reduced acceptor
-
?
all-trans-neurosporene + acceptor
-
Pantoea agglomerans all-trans-lycopene + reduced acceptor
-
?
all-trans-phytofluene + acceptor
-
Pantoea agglomerans all-trans-zeta-carotene + reduced acceptor
-
?
all-trans-zeta-carotene + acceptor
-
Pantoea agglomerans all-trans-neurosporene + reduced acceptor
-
?

Synonyms

Synonyms Comment Organism
CrtI
-
Pantoea agglomerans
phytoene desaturase
-
Pantoea agglomerans