Literature summary for 1.3.99.31 extracted from

Stickforth, P.; Sandmann, G.
Kinetic variations determine the product pattern of phytoene desaturase from Rubrivivax gelatinosus (2007), Arch. Biochem. Biophys., 461, 235-241.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli. Synthesis of the enzyme by different vectors with high and lower copy numbers or strong and weak promoters results in variable ratios of lycopene and neurosporene formation. The highest amount of enzyme is produced with pUC vectors with high copy number and a strong promoter. This transformant synthesizes the highest lycopene amount. The lowest enzyme concentrations is found with pPEU carrying a weak promoter. In this transformant lycopene formation is minor. With increased enzyme concentration, a proportion of lycopene higher than in Rubrivivax gelatinosus cells can be obtained	Rubrivivax gelatinosus

Cloned (Comment)

Organism

expression in Escherichia coli. Synthesis of the enzyme by different vectors with high and lower copy numbers or strong and weak promoters results in variable ratios of lycopene and neurosporene formation. The highest amount of enzyme is produced with pUC vectors with high copy number and a strong promoter. This transformant synthesizes the highest lycopene amount. The lowest enzyme concentrations is found with pPEU carrying a weak promoter. In this transformant lycopene formation is minor. With increased enzyme concentration, a proportion of lycopene higher than in Rubrivivax gelatinosus cells can be obtained

Rubrivivax gelatinosus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
15-cis-phytoene + 3 acceptor	Rubrivivax gelatinosus	-	all-trans-neurosporene + 3 reduced acceptor	-	?
15-cis-phytoene + 4 acceptor	Rubrivivax gelatinosus	-	all-trans-lycopene + 4 reduced acceptor	-	?

Organism

Organism	UniProt	Comment	Textmining
Rubrivivax gelatinosus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
15-cis-phytoene + 3 acceptor	-	Rubrivivax gelatinosus	all-trans-neurosporene + 3 reduced acceptor	-	?
15-cis-phytoene + 4 acceptor	-	Rubrivivax gelatinosus	all-trans-lycopene + 4 reduced acceptor	-	?

Synonyms

Synonyms	Comment	Organism
CrtI	-	Rubrivivax gelatinosus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
28	-	assay at	Rubrivivax gelatinosus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Rubrivivax gelatinosus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	added to the reaction mixture	Rubrivivax gelatinosus