Cloned (Comment) | Organism |
---|---|
gene pds, DNA and amino acid sequence determination and analysis of wild-type and mutant E17, expression of wild-type and mutants in Escherichia coli | Chromochloris zofingiensis |
Protein Variants | Comment | Organism |
---|---|---|
L516F | naturally occuring mutant strain E17, the mutant enzyme is insensitive, with 31fold higher resistane compared to the wild-tpe enzyme, to inhibition by norflurazon.The mutant also shows higher catalytic efficiency than the wild-type enzyme. Induced by high-light irradiation or glucose, E17 produces 44 or 36% more astaxanthin than the wild-type when cultured in media without norflurazon | Chromochloris zofingiensis |
L516R | the mutant show reduced enzyme activity compared to the wild-type enzyme | Chromochloris zofingiensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloro-5-(methylamino)-2-[3-(trifluoromethyl)phenyl]pyridazin-3(2H)-one | - |
Chromochloris zofingiensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + acceptor | Chromochloris zofingiensis | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | Chromochloris zofingiensis ATCC 30412 | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | Chromochloris zofingiensis | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | Chromochloris zofingiensis ATCC 30412 | - |
all-trans-zeta-carotene + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromochloris zofingiensis | A6N8C1 | - |
- |
Chromochloris zofingiensis ATCC 30412 | A6N8C1 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00032 | - |
recombinant mutant L516R, cell extracts of transformed Escherichia coli, pH 7.2, 28°C | Chromochloris zofingiensis |
0.0004 | - |
recombinant wild-type enzyme, cell extracts of transformed Escherichia coli, pH 7.2, 28°C | Chromochloris zofingiensis |
0.00053 | - |
recombinant mutant L516F, cell extracts of transformed Escherichia coli, pH 7.2, 28°C | Chromochloris zofingiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + acceptor | - |
Chromochloris zofingiensis | all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | - |
Chromochloris zofingiensis ATCC 30412 | all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + decyl plastoquinone | - |
Chromochloris zofingiensis | all-trans-phytofluene + decyl plastoquinol | - |
? | |
15-cis-phytoene + decyl plastoquinone | - |
Chromochloris zofingiensis ATCC 30412 | all-trans-phytofluene + decyl plastoquinol | - |
? | |
all-trans-phytofluene + acceptor | - |
Chromochloris zofingiensis | all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | - |
Chromochloris zofingiensis ATCC 30412 | all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + decyl plastoquinone | - |
Chromochloris zofingiensis | all-trans-zeta-carotene + decyl plastoquinol | - |
? | |
all-trans-phytofluene + decyl plastoquinone | - |
Chromochloris zofingiensis ATCC 30412 | all-trans-zeta-carotene + decyl plastoquinol | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Chromochloris zofingiensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Chromochloris zofingiensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
decyl-plastoquinone | - |
Chromochloris zofingiensis |
General Information | Comment | Organism |
---|---|---|
metabolism | carotenoid profiles of wild-type and mutant E17 strains, overview | Chromochloris zofingiensis |