BRENDA - Enzyme Database
show all sequences of 1.3.99.18

XVIII. Purification and some properties of the molybdenum- and iron-containing quinaldic acid 4-oxidoreductase from Serratia marcescens 2CC-1

Fetzner, S.; Lingens, F.; Biol. Chem. Hoppe-Seyler 374, 363-376 (1993)

Data extracted from this reference:

General Stability
General Stability
Organism
ethylene glycol, 0.1%, Triton X-10, 0.1%, and Tween, 0.01%, inactivate the enzyme within 1-2 days at 4 C
Serratia marcescens
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Serratia marcescens
Hg2+
-
Serratia marcescens
KCN
-
Serratia marcescens
methanol
-
Serratia marcescens
Mn2+
-
Serratia marcescens
NaAsO2
-
Serratia marcescens
p-hydroxymercuribenzoate
-
Serratia marcescens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.085
-
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
with quinaldate as cosubstrate
Serratia marcescens
1.45
-
Quinaldate
with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate
Serratia marcescens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
enzyme contains 2.3-3.7 gatom of iron per mol of enzyme
Serratia marcescens
Molybdenum
enzyme contains 0.5-0.6 gatom of molybdenum per mol of enzyme
Serratia marcescens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
90000
100000
gel filtration
Serratia marcescens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Quinaldate + acceptor
Serratia marcescens
inducible enzyme
?
-
-
-
Quinaldate + acceptor
Serratia marcescens 2CC-1
inducible enzyme
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Serratia marcescens
-
2CC-1
-
Serratia marcescens 2CC-1
-
2CC-1
-
Purification (Commentary)
Commentary
Organism
-
Serratia marcescens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
64.5
-
-
Serratia marcescens
Storage Stability
Storage Stability
Organism
-80°C, 8 days, 25% loss of activity
Serratia marcescens
4°C, 8 days, 30% loss of activity
Serratia marcescens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
nicotinic acid + acceptor
-
12576
Serratia marcescens
6-hydroxynicotinic acid + reduced acceptor
-
12576
Serratia marcescens
-
nicotinic acid + acceptor
-
12576
Serratia marcescens 2CC-1
6-hydroxynicotinic acid + reduced acceptor
-
12576
Serratia marcescens 2CC-1
-
quinaldate + acceptor
acceptor: K3[Fe(CN)6]
12576
Serratia marcescens
kynurenate + reduced acceptor
-
-
-
-
quinaldate + acceptor
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
12576
Serratia marcescens
kynurenate + reduced acceptor
-
-
-
-
quinaldate + acceptor
acceptor: K3[Fe(CN)6]
12576
Serratia marcescens 2CC-1
kynurenate + reduced acceptor
-
-
-
-
quinaldate + acceptor
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
12576
Serratia marcescens 2CC-1
kynurenate + reduced acceptor
-
-
-
-
Quinaldate + acceptor
inducible enzyme
12576
Serratia marcescens
?
-
-
-
-
Quinaldate + acceptor
inducible enzyme
12576
Serratia marcescens 2CC-1
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
1 * 18000-19000, beta, + 1 * 75000-80000, alpha, SDS-PAGE
Serratia marcescens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
70
-
-
Serratia marcescens
General Stability (protein specific)
General Stability
Organism
ethylene glycol, 0.1%, Triton X-10, 0.1%, and Tween, 0.01%, inactivate the enzyme within 1-2 days at 4 C
Serratia marcescens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Serratia marcescens
Hg2+
-
Serratia marcescens
KCN
-
Serratia marcescens
methanol
-
Serratia marcescens
Mn2+
-
Serratia marcescens
NaAsO2
-
Serratia marcescens
p-hydroxymercuribenzoate
-
Serratia marcescens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.085
-
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
with quinaldate as cosubstrate
Serratia marcescens
1.45
-
Quinaldate
with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate
Serratia marcescens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
enzyme contains 2.3-3.7 gatom of iron per mol of enzyme
Serratia marcescens
Molybdenum
enzyme contains 0.5-0.6 gatom of molybdenum per mol of enzyme
Serratia marcescens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
90000
100000
gel filtration
Serratia marcescens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Quinaldate + acceptor
Serratia marcescens
inducible enzyme
?
-
-
-
Quinaldate + acceptor
Serratia marcescens 2CC-1
inducible enzyme
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Serratia marcescens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
64.5
-
-
Serratia marcescens
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, 8 days, 25% loss of activity
Serratia marcescens
4°C, 8 days, 30% loss of activity
Serratia marcescens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
nicotinic acid + acceptor
-
12576
Serratia marcescens
6-hydroxynicotinic acid + reduced acceptor
-
12576
Serratia marcescens
-
nicotinic acid + acceptor
-
12576
Serratia marcescens 2CC-1
6-hydroxynicotinic acid + reduced acceptor
-
12576
Serratia marcescens 2CC-1
-
quinaldate + acceptor
acceptor: K3[Fe(CN)6]
12576
Serratia marcescens
kynurenate + reduced acceptor
-
-
-
-
quinaldate + acceptor
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
12576
Serratia marcescens
kynurenate + reduced acceptor
-
-
-
-
quinaldate + acceptor
acceptor: K3[Fe(CN)6]
12576
Serratia marcescens 2CC-1
kynurenate + reduced acceptor
-
-
-
-
quinaldate + acceptor
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
12576
Serratia marcescens 2CC-1
kynurenate + reduced acceptor
-
-
-
-
Quinaldate + acceptor
inducible enzyme
12576
Serratia marcescens
?
-
-
-
-
Quinaldate + acceptor
inducible enzyme
12576
Serratia marcescens 2CC-1
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
1 * 18000-19000, beta, + 1 * 75000-80000, alpha, SDS-PAGE
Serratia marcescens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
70
-
-
Serratia marcescens
Other publictions for EC 1.3.99.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
12575
Sauter
XX. Quinaldic acid 4-oxidoredu ...
Pseudomonas sp., Pseudomonas sp. AK-2
Biol. Chem. Hoppe-Seyler
374
1037-1046
1993
-
-
-
-
-
-
11
2
-
2
4
-
-
7
-
-
1
-
-
-
1
-
8
1
1
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
11
-
2
-
2
4
-
-
-
-
1
-
-
1
-
8
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
12576
Fetzner
XVIII. Purification and some p ...
Serratia marcescens 2CC-1, Serratia marcescens
Biol. Chem. Hoppe-Seyler
374
363-376
1993
-
-
-
-
-
1
7
2
-
2
1
2
-
6
-
-
1
-
-
-
1
2
8
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
7
-
2
-
2
1
2
-
-
-
1
-
-
1
2
8
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-