Cloned (Comment) | Organism |
---|---|
into vector pUF1, expression in Escherichia coli DH5alpha pBL9 | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
A259G | turnover rate 2.4fold reduced, no significant altered Km | Pseudomonas putida |
A546G | turnover rate 199fold reduced, increased affinity for quinoline and quinoxaline | Pseudomonas putida |
F375V | as stable as wild-type | Pseudomonas putida |
V373A | turnover rate 40fold reduced | Pseudomonas putida |
V373Y | as stable as wild-type | Pseudomonas putida |
W331G | turnover rate 38fold reduced, suitable for hydroxylation of quinoxaline and C4-substituted quinolines | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
Quinoline | mutant A546G | Pseudomonas putida | |
0.12 | - |
Quinoline | purified Qor protein | Pseudomonas putida | |
0.13 | - |
Quinoline | mutant A259G | Pseudomonas putida | |
0.33 | - |
Quinoline | mutant W331G | Pseudomonas putida | |
0.41 | - |
Quinoline | mutant V373A | Pseudomonas putida | |
0.68 | - |
Quinoxaline | mutantA546G | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida 86-1deltaqor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to electrophoretic homogeneity | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.08 | - |
mutant A546G with quinoline as substrate | Pseudomonas putida |
0.36 | - |
mutant W331G with quinoline as substrate | Pseudomonas putida |
0.57 | - |
mutant V373A with quinoline as substrate | Pseudomonas putida |
16 | - |
mutant A259G with quinoline as substrate | Pseudomonas putida |
22 | - |
purified Qor protein with quinoline as substrate | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
3-methylquinoline + acceptor + H2O | - |
Pseudomonas putida 86-1deltaqor | ? | - |
? | |
4-chloroquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
4-chloroquinoline + acceptor + H2O | - |
Pseudomonas putida 86-1deltaqor | ? | - |
? | |
4-hydroxyquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
4-hydroxyquinoline + acceptor + H2O | - |
Pseudomonas putida 86-1deltaqor | ? | - |
? | |
4-methylquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
4-methylquinoline + acceptor + H2O | - |
Pseudomonas putida 86-1deltaqor | ? | - |
? | |
5-carboxyquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
5-carboxyquinoline + acceptor + H2O | - |
Pseudomonas putida 86-1deltaqor | ? | - |
? | |
6-hydroxyquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
6-methylquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
7-hydroxyquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
8-hydroxyquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
8-methylquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
quinoline + acceptor + H2O | - |
Pseudomonas putida | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? | |
quinoxaline + acceptor + H2O | - |
Pseudomonas putida | 2-oxoquinoxaline + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Qor | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
Quinoline | mutant A546G | Pseudomonas putida | |
2.14 | - |
Quinoline | mutant V373A | Pseudomonas putida | |
2.2 | - |
Quinoxaline | mutantA546G | Pseudomonas putida | |
2.27 | - |
Quinoline | mutant W331G | Pseudomonas putida | |
35.7 | - |
Quinoline | mutant A259G | Pseudomonas putida | |
85.4 | - |
Quinoline | purified Qor protein | Pseudomonas putida |