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Literature summary for 1.3.98.3 extracted from

  • Kim, E.J.; Oh, E.K.; Lee, J.K.
    Role of HemF and HemN in the heme biosynthesis of Vibrio vulnificus under S-adenosylmethionine-limiting conditions (2015), Mol. Microbiol., 96, 497-512.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Vibrio vulnificus

Protein Variants

Protein Variants Comment Organism
Y74F the apparent binding affinity of the mutant enzyme for the cofactor S-adenosyl-L-methionine is higher than that of the wild type enzyme Vibrio vulnificus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0052
-
Coproporphyrinogen III wild type enzyme, at pH 8.0 and 30°C Vibrio vulnificus
0.0067
-
Coproporphyrinogen III mutant enzyme Y74F, at pH 8.0 and 30°C Vibrio vulnificus
0.15
-
S-adenosyl-L-methionine mutant enzyme Y74F, at pH 8.0 and 30°C Vibrio vulnificus
0.42
-
S-adenosyl-L-methionine wild type enzyme, at pH 8.0 and 30°C Vibrio vulnificus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
-
Vibrio vulnificus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coproporphyrinogen III + S-adenosyl-L-methionine Vibrio vulnificus
-
protoporphyrinogen IX + CO2 + L-methionine + 5'-deoxyadenosine
-
?
coproporphyrinogen III + S-adenosyl-L-methionine Vibrio vulnificus ATCC 29307
-
protoporphyrinogen IX + CO2 + L-methionine + 5'-deoxyadenosine
-
?

Organism

Organism UniProt Comment Textmining
Vibrio vulnificus
-
-
-
Vibrio vulnificus ATCC 29307
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-Tactin-Sepharose column chromatography Vibrio vulnificus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coproporphyrinogen III + S-adenosyl-L-methionine
-
Vibrio vulnificus protoporphyrinogen IX + CO2 + L-methionine + 5'-deoxyadenosine
-
?
coproporphyrinogen III + S-adenosyl-L-methionine
-
Vibrio vulnificus ATCC 29307 protoporphyrinogen IX + CO2 + L-methionine + 5'-deoxyadenosine
-
?

Subunits

Subunits Comment Organism
? x * 55000, Strep-tagged enzyme, SDS-PAGE Vibrio vulnificus

Synonyms

Synonyms Comment Organism
coproporphyrinogen III oxidase
-
Vibrio vulnificus
CPO
-
Vibrio vulnificus
HemN
-
Vibrio vulnificus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
360
-
Coproporphyrinogen III mutant enzyme Y74F, at pH 8.0 and 30°C Vibrio vulnificus
900
-
Coproporphyrinogen III wild type enzyme, at pH 8.0 and 30°C Vibrio vulnificus

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center
-
Vibrio vulnificus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
54000
-
Coproporphyrinogen III mutant enzyme Y74F, at pH 8.0 and 30°C Vibrio vulnificus
175000
-
Coproporphyrinogen III wild type enzyme, at pH 8.0 and 30°C Vibrio vulnificus