Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Vibrio vulnificus |
Protein Variants | Comment | Organism |
---|---|---|
Y74F | the apparent binding affinity of the mutant enzyme for the cofactor S-adenosyl-L-methionine is higher than that of the wild type enzyme | Vibrio vulnificus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0052 | - |
Coproporphyrinogen III | wild type enzyme, at pH 8.0 and 30°C | Vibrio vulnificus | |
0.0067 | - |
Coproporphyrinogen III | mutant enzyme Y74F, at pH 8.0 and 30°C | Vibrio vulnificus | |
0.15 | - |
S-adenosyl-L-methionine | mutant enzyme Y74F, at pH 8.0 and 30°C | Vibrio vulnificus | |
0.42 | - |
S-adenosyl-L-methionine | wild type enzyme, at pH 8.0 and 30°C | Vibrio vulnificus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
- |
Vibrio vulnificus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen III + S-adenosyl-L-methionine | Vibrio vulnificus | - |
protoporphyrinogen IX + CO2 + L-methionine + 5'-deoxyadenosine | - |
? | |
coproporphyrinogen III + S-adenosyl-L-methionine | Vibrio vulnificus ATCC 29307 | - |
protoporphyrinogen IX + CO2 + L-methionine + 5'-deoxyadenosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio vulnificus | - |
- |
- |
Vibrio vulnificus ATCC 29307 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Strep-Tactin-Sepharose column chromatography | Vibrio vulnificus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen III + S-adenosyl-L-methionine | - |
Vibrio vulnificus | protoporphyrinogen IX + CO2 + L-methionine + 5'-deoxyadenosine | - |
? | |
coproporphyrinogen III + S-adenosyl-L-methionine | - |
Vibrio vulnificus ATCC 29307 | protoporphyrinogen IX + CO2 + L-methionine + 5'-deoxyadenosine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, Strep-tagged enzyme, SDS-PAGE | Vibrio vulnificus |
Synonyms | Comment | Organism |
---|---|---|
coproporphyrinogen III oxidase | - |
Vibrio vulnificus |
CPO | - |
Vibrio vulnificus |
HemN | - |
Vibrio vulnificus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
360 | - |
Coproporphyrinogen III | mutant enzyme Y74F, at pH 8.0 and 30°C | Vibrio vulnificus | |
900 | - |
Coproporphyrinogen III | wild type enzyme, at pH 8.0 and 30°C | Vibrio vulnificus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S]-center | - |
Vibrio vulnificus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
54000 | - |
Coproporphyrinogen III | mutant enzyme Y74F, at pH 8.0 and 30°C | Vibrio vulnificus | |
175000 | - |
Coproporphyrinogen III | wild type enzyme, at pH 8.0 and 30°C | Vibrio vulnificus |