Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | added to the assay at 2 mM | Synechocystis sp. | |
NADH | added to the assay at 0.5 mM | Synechocystis sp. |
Cloned (Comment) | Organism |
---|---|
genes sll1876 and sll1917, overexpression of N-terminally Strep-tagged HemNs in Escherichia coli | Synechocystis sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of deletion mutants of the HemN genes, growth of the mutant DELTAsll1876 is significantly slower than that of the wild-type under microoxic conditions, while it grows normally under aerobic conditions. Coproporphyrin III is accumulated at a low but significant level in the DELTAsll1876 mutant grown under micro-oxic conditions. No detectable phenotype in DELTAsll1917 under the conditions, phenotypes, overview | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen III + 2 S-adenosyl-L-methionine | Synechocystis sp. | sll1876 encodes HemN operating under micro-oxic conditions | protoporphyrinogen IX + 2 CO2 + 2 L-methionine + 2 5'-deoxyadenosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
two hemN genes, sll1876 and sll1917 | - |
Purification (Comment) | Organism |
---|---|
recombinant terminally Strep-tagged HemNs from Escherichia coli to homogeneity | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen III + 2 S-adenosyl-L-methionine | sll1876 encodes HemN operating under micro-oxic conditions | Synechocystis sp. | protoporphyrinogen IX + 2 CO2 + 2 L-methionine + 2 5'-deoxyadenosine | - |
? | |
coproporphyrinogen III + 2 S-adenosyl-L-methionine | only Sll1876 shows CPO activity under anaerobic conditions, Sll1917 is inactive | Synechocystis sp. | protoporphyrinogen IX + 2 CO2 + 2 L-methionine + 2 5'-deoxyadenosine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
coproporphyrinogen III oxidase | - |
Synechocystis sp. |
CPO | - |
Synechocystis sp. |
Sll1876 | - |
Synechocystis sp. |
Sll1917 | - |
Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
iron-sulfur centre | in Sll1876 and Sll1917 | Synechocystis sp. | |
S-adenosyl-L-methionine | - |
Synechocystis sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | catalyzes the decarboxylation of coproporphyrinogen III to form protoporphyrinogen IX in heme biosynthesis and is shared in chlorophyll biosynthesis in photosynthetic organisms | Synechocystis sp. |