Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.98.1 extracted from

  • Wolfe, A.E.; Thymark, M.; Gattis, S.G.; Fagan, R.L.; Hu, Y.C.; Johansson, E.; Arent, S.; Larsen, S.; Palfey, B.A.
    Interaction of benzoate pyrimidine analogues with class 1A dihydroorotate dehydrogenase from Lactococcus lactis (2007), Biochemistry, 46, 5741-5753.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Lactococcus lactis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, with 30% PEG 6000, 1 mM dithiothreitol, 0.2 M sodium acetate, and 0.1 M Tris-HCl at pH 8.5 Lactococcus lactis

Protein Variants

Protein Variants Comment Organism
C130A the mutant forms charge-transfer complexes upon binding 3,4-dihydroxybenzoate, but the maximum of the broad charge-transfer bands is shifted to 590 nm Lactococcus lactis
C130S the mutant forms charge-transfer complexes upon binding 3,4-dihydroxybenzoate, but the maximum of the broad charge-transfer bands is shifted to 610 nm Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
3,4-dihydroxybenzoate
-
Lactococcus lactis
3,5-Dihydroxybenzoate
-
Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroorotate + 2,6-dichlorophenolindophenol
-
Lactococcus lactis orotate + reduced 2,6-dichlorophenolindophenol
-
?

Synonyms

Synonyms Comment Organism
class 1A DHOD
-
Lactococcus lactis
class 1A dihydroorotate dehydrogenase
-
Lactococcus lactis