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Literature summary for 1.3.98.1 extracted from

  • Fagan, R.L.; Jensen, K.F.; Bjoernberg, O.; Palfey, B.A.
    Mechanism of flavin reduction in the class 1A dihydroorotate dehydrogenase from Lactococcus lactis (2007), Biochemistry, 46, 4028-4036.
    View publication on PubMed
Search for more literature for 1.3.98.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in SO6645 cells Lactococcus lactis

Protein Variants

Protein Variants Comment Organism
C130A the mutant enzyme exhibits binding affinities for dihydroorotate similar to that of the wild type enzyme, reduction is extremely slow compared to that of the wild type, the rate of reduction increases with pH showing no sign of a plateau Lactococcus lactis
C130S the mutant enzyme exhibits binding affinities for dihydroorotate similar to that of the wild type enzyme, reduction is extremely slow compared to that of the wild type, the rate of reduction increases with pH showing no sign of a plateau Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Q-Sepharose column chromatography Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-dihydroorotate + fumarate
-
 Lactococcus lactis orotate + succinate
-
 ?

Synonyms

Synonyms Comment Organism
class 1A DHOD
-
 Lactococcus lactis
class 1A dihydroorotate dehydrogenase
-
 Lactococcus lactis
DHODH
-
 Lactococcus lactis

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Lactococcus lactis