Literature summary for 1.3.98.1 extracted from

Inaoka, D.K.; Takashima, E.; Osanai, A.; Shimizu, H.; Nara, T.; Aoki, T.; Harada, S.; Kita, K.
Expression, purification and crystallization of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with orotate (2005), Acta Crystallogr. Sect. F, 61, 875-878.

Activating Compound

Activating Compound	Comment	Organism	Structure
IPTG	induces expression	Trypanosoma cruzi

Cloned(Commentary)

Cloned (Comment)	Organism
ligated into the pZErO-2 vector and transformation of the Escherichia coli strain TOP 10, inserted into the pET3a expression vector and transformation of Escherichia coli BL21	Trypanosoma cruzi

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of the TcDHODorotate complex are grown at 4°C by the sitting-drop vapour-diffusion technique using polyethylene glycol 3350 as a precipitant. The crystals diffract to better than 1.8 A ° resolution using synchrotron radiation (lambda = 0.900 A). X-ray diffraction data are collected at -173°C and processed to 1.9 A ° resolution with 98.2% completeness. The TcDHOD crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.87, b = 71.89, c = 123.27 A	Trypanosoma cruzi
DHODorotate complex by the sitting-drop vapour-diffusion technique using polyethylene glycol 3350 as a precipitant, to better than 1.8 A resolution, crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 67.87, b = 71.89, c = 123.27 A	Trypanosoma cruzi

Crystallization (Comment)

Organism

crystals of the TcDHODorotate complex are grown at 4°C by the sitting-drop vapour-diffusion technique using polyethylene glycol 3350 as a precipitant. The crystals diffract to better than 1.8 A ° resolution using synchrotron radiation (lambda = 0.900 A). X-ray diffraction data are collected at -173°C and processed to 1.9 A ° resolution with 98.2% completeness. The TcDHOD crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.87, b = 71.89, c = 123.27 A

Trypanosoma cruzi

DHODorotate complex by the sitting-drop vapour-diffusion technique using polyethylene glycol 3350 as a precipitant, to better than 1.8 A resolution, crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 67.87, b = 71.89, c = 123.27 A

Trypanosoma cruzi

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma cruzi	-	-	-
Trypanosoma cruzi	Q4D3W2	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant	Trypanosoma cruzi
to homogeneity by ion-exchange, hydrophobic interaction and gel filtration	Trypanosoma cruzi

Synonyms

Synonyms	Comment	Organism
DHOD2	-	Trypanosoma cruzi
TcDHOD	-	Trypanosoma cruzi