Literature summary for 1.3.98.1 extracted from

Hurt, D.E.; Widom, J.; Clardy, J.
Structure of Plasmodium falciparum dihydroorotate dehydrogenase with a bound inhibitor (2006), Acta Crystallogr. Sect. D, 62, 312-323.

Search for more literature for 1.3.98.1

Application

Application	Comment	Organism
additional information	species-specific preferential inhibitor binding, different binding mode for the same inhibitor in the two catalytically identical enzymes human DHODH and Plasmodium DHODH	Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment)	Organism
into vector pRSET and expressed in either freshly transformed or glycerol stocked Escherichia coli BL21-CodonPlus (DE3)-RIL	Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment)	Organism
by sitting-drop vapor-diffusion technique, to 2.4 A resolution	Plasmodium falciparum

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	brequinar and DPC-AE661100 have no effect, even at a concentration of 1 mM	Plasmodium falciparum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0131	-	2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone	at saturating concentration of dihydroorotate	Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	gel filtration	Plasmodium falciparum

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	Q08210	synthetic construct	-

Purification (Commentary)

Purification (Comment)	Organism
soniciation and gel filtration	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dihydroorotate + 2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone	-	Plasmodium falciparum	orotate + reduced 2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone	-	?
dihydroorotate + 2,6-dichloroindophenol	-	Plasmodium falciparum	orotate + reduced 2,6-dichloroindophenol	-	?

Synonyms

Synonyms	Comment	Organism
DHODH	-	Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone	at saturating concentration of dihydroorotate	Plasmodium falciparum

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Release 2023.1 (January 2023)