Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-thioorotate | orotate analogue, 50% inhibition at 0.018 mM | Saccharomyces cerevisiae | |
5-aminoorotate | orotate analogue, 50% inhibition at 1.6 mM | Saccharomyces cerevisiae | |
5-bromoorotate | orotate analogue, 50% inhibition at 0.016 mM | Saccharomyces cerevisiae | |
5-Fluoroorotate | orotate analogue, 50% inhibition at 0.055 mM | Saccharomyces cerevisiae | |
5-nitroorotate | orotate analogue, 50% inhibition at 0.076 mM | Saccharomyces cerevisiae | |
Orotate | competitive, with ferricyanide as electron acceptor | Saccharomyces cerevisiae | |
orotate methyl ester | orotate analogue, 50% inhibition at 0.71 mM | Saccharomyces cerevisiae | |
oxonic acid | orotate analogue, 50% inhibition at 0.0099 mM | Saccharomyces cerevisiae | |
Uracil | orotate analogue, 50% inhibition at 2.5 mM | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
S-dihydroorotate | with O2 | Saccharomyces cerevisiae | |
0.0084 | 0.045 | S-dihydroorotate | with fumarate | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
72000 | - |
sucrose-density-gradient centrifugation | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + acceptor | Saccharomyces cerevisiae | fourth step in synthesis of pyrimidine nucleotides | orotate + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae | - |
family 1 enzyme A | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Orange-A column, reverse-affinity step, Mono Q column | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + acceptor | preferred electron acceptors in the decreasing order: ferricyanide, 2,6-dichlorophenolindophenol, ubiquinone-0, fumarate and O2 | Saccharomyces cerevisiae | orotate + reduced acceptor | - |
? | |
(S)-dihydroorotate + acceptor | fourth step in synthesis of pyrimidine nucleotides | Saccharomyces cerevisiae | orotate + reduced acceptor | - |
? | |
dihydroorotate + acceptor | acceptor: 2,6-dichlorophenolindophenol | Saccharomyces cerevisiae | orotate + reduced acceptor | - |
? | |
dihydroorotate + acceptor | kinetic isotope effects | Saccharomyces cerevisiae | orotate + reduced acceptor | - |
? | |
dihydroorotate + acceptor | acceptor: ferricyanide, coenzyme Q0, fumarate, molecular oxygen | Saccharomyces cerevisiae | orotate + reduced acceptor | - |
? | |
dihydrooxonate + acceptor | - |
Saccharomyces cerevisiae | oxonate + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
DHOD | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.8 | - |
S-dihydroorotate | with O2 | Saccharomyces cerevisiae | |
23 | 25 | S-dihydroorotate | with fumarate, pH 7.5 | Saccharomyces cerevisiae | |
39 | - |
dihydrooxonate | with ferricyanide | Saccharomyces cerevisiae | |
190 | - |
S-dihydroorotate | with ferricyanide | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | flavoprotein, 1 FMN per subunit | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0102 | - |
Orotate | pH 8.0 | Saccharomyces cerevisiae |